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Terbium nanoparticle biofunctionalization for extracellular biosensing

Abstract : Lanthanide-doped nanoparticles (LnNPs) have become an important class of fluorophores for advanced biosensing and bioimaging. However, developing bright, stable, and bioconjugated LnNPs remains an important challenge for their translation into functional bioanalytical applications. Here, we present in-situ detection of ligand-receptor binding on cells, which was accomplished with TbNP-antibody (Matuzumab) conjugates that could specifically bind to transmembrane epidermal growth factor receptors (EGFR). High specificity and sensitivity were shown by time-gated imaging of EGFR on both strongly (A431) and weakly (HeLa and Cos7) EGFR-expressing cell lines, whereas non-expressing cells did not show any signals. Despite the relatively large size of TbNP-antibody conjugates, they could be internalized by A431 cells upon binding to extracellular EGFR, which showed their potential as bright and stable luminescence markers for intracellular signaling. Stability tests of TbNPs over a range of pH values, different buffers, over time, and sonication effect on these NPs were performed by using dynamic light scattering (DLS) and zeta potential (ZP) measurements.
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Vjona Çifliku. Terbium nanoparticle biofunctionalization for extracellular biosensing. Inorganic chemistry. Université Paris-Saclay, 2020. English. ⟨NNT : 2020UPASS075⟩. ⟨tel-02938737⟩

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