Speciation of radionuclides with bioligands using time-resolved laser-induced fluorescence (TRLIF) and electrospray mass spectrometry (ES-MS)

V. Lourenco (1, 2) , E. Ansoborlo (1, 3) , G. Cote (4) , C. Moulin (1, 2)

1 CEA-DES (ex-DEN) - CEA-Direction des Energies (ex-Direction de l'Energie Nucléaire)
2 DPC - Département de Physico-Chimie
3 CETAMA - Commission des méthodes d'analyse du CEA
4 ENSCP - Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL

Studies on radionuclides complexation and uptake mechanisms at the cellular and molecular level are very scarce. Consequently, the chelating properties of these elements in biological media are not fully understood. As a result, we aim at investigating the interactions between the radionuclide cation and several bioligands.The selected systems were investigated by two major analytical techniques, namely electrospray mass spectrometry (ES-MS) as well as time-resolved emission spectroscopy (TRES). These two techniques are very powerful for speciation studies as, aside their speciation capability, they also allow to determine interaction constants. Moreover, as spectroscopic techniques, they can lead to spectral databanks that are very useful when starting from simple to more complex media. To confirm the obtained results, intercomparison studies were performed together with molecular modelling. The experiments carried out by TRES and ES-MS will be presented.

Keywords: speciation; mass spectrometry; radionuclides; time resolved laser induced fluorescence

Domains: Nuclear Experiment [nucl-ex]; Nuclear Theory [nucl-th]

V. Lourenco, E. Ansoborlo, G. Cote, C. Moulin. Speciation of radionuclides with bioligands using time-resolved laser-induced fluorescence (TRLIF) and electrospray mass spectrometry (ES-MS). R. Alvares; N. D. Bryan; I. May. Recent Advances in Actinide Science, RSC Publishing, pp.785-787, 2006, ⟨10.1039/9781847555366-00011⟩. ⟨cea-02355785⟩