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Slow magic-angle coil spinning: A high-sensitivity and high-resolution NMR strategy for microscopic biological specimens

Alan Wong 1 Pedro M. Aguiar 1 Dimitris Sakellariou 1, *
* Corresponding author
1 LSDRM - Laboratoire Structure et Dynamique par Résonance Magnétique (LCF)
NIMBE UMR 3685 - Nanosciences et Innovation pour les Matériaux, la Biomédecine et l'Energie (ex SIS2M)
Abstract : High-resolution magic-angle spinning has become a valuable tool to study biologic samples; however, there are only a few high-resolution magic-angle spinning methodologies that have been explored specifically to deal with small semisolid biomaterials such as tissues. Here, we report a novel high-resolution 1H NMR spectroscopic approach using magic-angle coil spinning. We demonstrate its potential for intact tissues by combining a resonant microcoil with a large-volume commercial magic-angle spinning rotor, where the microcoil offers incredible sensitivity and the large-volume magic-angle spinning rotor provides stable slow magic-angle spinning. This approach provides high-resolution spectra with high sensitivity and the preservation of living biologic specimens for an accurate chemical analysis.
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https://hal-cea.archives-ouvertes.fr/cea-00854518
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Submitted on : Tuesday, August 27, 2013 - 2:34:22 PM
Last modification on : Monday, February 10, 2020 - 6:12:48 PM

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Alan Wong, Pedro M. Aguiar, Dimitris Sakellariou. Slow magic-angle coil spinning: A high-sensitivity and high-resolution NMR strategy for microscopic biological specimens. Magnetic Resonance in Medicine, Wiley, 2010, 63, pp.269-274. ⟨10.1002/mrm.22231⟩. ⟨cea-00854518⟩

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