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Article Dans Une Revue Magnetic Resonance in Medicine Année : 2010

Slow magic-angle coil spinning: A high-sensitivity and high-resolution NMR strategy for microscopic biological specimens

Résumé

High-resolution magic-angle spinning has become a valuable tool to study biologic samples; however, there are only a few high-resolution magic-angle spinning methodologies that have been explored specifically to deal with small semisolid biomaterials such as tissues. Here, we report a novel high-resolution 1H NMR spectroscopic approach using magic-angle coil spinning. We demonstrate its potential for intact tissues by combining a resonant microcoil with a large-volume commercial magic-angle spinning rotor, where the microcoil offers incredible sensitivity and the large-volume magic-angle spinning rotor provides stable slow magic-angle spinning. This approach provides high-resolution spectra with high sensitivity and the preservation of living biologic specimens for an accurate chemical analysis.

Dates et versions

cea-00854518 , version 1 (27-08-2013)

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Citer

Alan Wong, Pedro M. Aguiar, Dimitris Sakellariou. Slow magic-angle coil spinning: A high-sensitivity and high-resolution NMR strategy for microscopic biological specimens. Magnetic Resonance in Medicine, 2010, 63, pp.269-274. ⟨10.1002/mrm.22231⟩. ⟨cea-00854518⟩
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