Optimization of the harvesting and freezing conditions of human cell lines for DNA damage analysis by the alkaline comet assay

Maria João Bessa (1) , Fátima Brandão (1) , Micaela Machado Querido (1) , Carla Costa (1) , Cristiana Costa Pereira (1) , Vanessa Valdiglesias (2) , Blanca Laffon (2) , Marie Carrière (3, 4) , João Paulo Teixeira (1) , Sónia Fraga (1)

1 INSA - Instituto Nacional de Saùde Dr Ricardo Jorge [Portugal]
2 Universidade da Coruña
3 CIBEST - Chimie Interface Biologie pour l’Environnement, la Santé et la Toxicologie
4 DIESE - Département Interfaces pour l'énergie, la Santé et l'Environnement

Domaines: Sciences du Vivant [q-bio]; Toxicologie

Maria João Bessa, Fátima Brandão, Micaela Machado Querido, Carla Costa, Cristiana Costa Pereira, et al.. Optimization of the harvesting and freezing conditions of human cell lines for DNA damage analysis by the alkaline comet assay. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2019, 845, pp.402994. ⟨10.1016/j.mrgentox.2018.12.002⟩. ⟨hal-03111813⟩