Division-Based, Growth Rate Diversity in Bacteria

Ghislain Y. Gangwe Nana (1) , Camille Ripoll (2) , Armelle Cabin-Flaman (3) , D. Gibouin (3) , Anthony Delaune (3) , Laurent Jannière (4) , Gerard Grancher (5) , Gaëlle Chagny (5) , Corinne Loutelier-Bourhis (6) , Esther Lentzen (7) , Patrick Grysan (8) , Jean-Nicolas Audinot (8) , Vic Norris (1)

1 LMSM - Laboratoire de Microbiologie Signaux et Microenvironnement
2 UNIROUEN - Université de Rouen Normandie
3 GPM - Groupe de physique des matériaux
4 ISSB - Institut de biologie systémique et synthétique
5 LMRS - Laboratoire de Mathématiques Raphaël Salem
6 COBRA - Chimie Organique et Bioorganique : Réactivité et Analyse
7 LIST - Luxembourg Institute of Science and Technology
8 Materials Research and Technology (MRT) Department

To investigate the nature and origins of growth rate diversity in bacteria, we grew Escherichia coli and Bacillus subtilis in liquid minimal media and, after different periods of ¹⁵N-labeling, analyzed and imaged isotope distributions in individual cells with Secondary Ion Mass Spectrometry. We find a striking inter- and intra-cellular diversity, even in steady state growth. This is consistent with the strand-dependent, hyperstructure-based hypothesis that a major function of the cell cycle is to generate coherent, growth rate diversity via the semi-conservative pattern of inheritance of strands of DNA and associated macromolecular assemblies. We also propose quantitative, general, measures of growth rate diversity for studies of cell physiology that include antibiotic resistance.

Mots clés: bacteria; DNA segregation; cell cycle; secondary Ion mass spectrometry; asymmetry; heterogeneity; NanoSIMS 50; isotope-labeling

Domaines: Bactériologie

Ghislain Y. Gangwe Nana, Camille Ripoll, Armelle Cabin-Flaman, D. Gibouin, Anthony Delaune, et al.. Division-Based, Growth Rate Diversity in Bacteria. Frontiers in Microbiology, 2018, 9, pp.849. ⟨10.3389/fmicb.2018.00849⟩. ⟨hal-02158528⟩