Etude des signaux bystander émis par des cellules de cartilage cultivées en 3D et irradiées in vitro dans un contexte de radiothérapie conventionnelle et d'Hadronthérapie

Abstract : In conventional radiotherapy (X-ray) or unconventional radiotherapy (Hadrontherapy), the impact of irradiation on healthy tissue raises essential questions of radiobiology, these healthy tissues being in the path of the beam during the treatment of a patient. tumor. Among these questions, what is the impact of the radio-induced Bystander effect? This mechanism involves stress signals still poorly identified, emitted by one or more irradiated cells to adjacent or very close non-irradiated cells, which can produce biological effects close to those obtained in the irradiated zone.To study this phenomenon, we used various molecular biology techniques including: clonogenic assay to study the survival fraction of cells after treatment, two-dimensional electrophoresis allowing the analysis of the cellular proteome, conditioned medium analysis for identify Bystander signals emitted by irradiated cells and proteomembrane experiments aimed at studying cells in a mix. In parallel with these study techniques, we have developed a 3D cell culture model via the use of "pellets" (cell aggregates).Our results showed a decrease in the survival of bystander cells after transfer of medium from irradiated cells, the potential involvement of some cytokines in bystander signaling as well as several candidate proteins that may partly explain the bystander response.
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Charlotte Lepleux. Etude des signaux bystander émis par des cellules de cartilage cultivées en 3D et irradiées in vitro dans un contexte de radiothérapie conventionnelle et d'Hadronthérapie. Biologie cellulaire. Normandie Université, 2018. Français. ⟨NNT : 2018NORMC289⟩. ⟨tel-02293058⟩

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