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Biocapteurs à fibres optiques fondés sur les réseaux de Bragg en angle fonctionnalisés

Séverine Maguis 1
1 LCFO - Laboratoire Capteurs Fibres Optiques
DM2I - Département Métrologie Instrumentation & Information : DRT/LIST/DM2I
Abstract : The purpose of this research work is to investigate the possibility of using tilted fiber Bragg grating technology to provide an alternative solution for molecular detection in aqueous solutions, suitable for in situ measurements and simpler to use than conventional monitoring instruments. As a tilted …ber Bragg grating is sensitive to any change in the refractive index of the medium surrounding the cladding, this refractometer can be used as a transducer for biosensing applications if the silica …ber surface is biofunctionalized. One of the great challenges to be met in the preparation of biosensors is the strategy for immobilization of the biospeci…c molecule onto the transducer. The binding of the target molecule to the immobilized probe results in changes in the thickness and/or the refractive index of the biofilm, which modulates the spectral response of the grating. The sensitive area of the refractometer combines two complementary functions: on the one hand, the biospeci…c biofilm enables one to detect the target molecule by converting binding events into physical changes of the sensitive layer; on the other hand, the tilted …ber Bragg grating translates this thickness / refractive index modi…cation into a spectral modulation. Bovine serum albumin (BSA) and anti-BSA are used to assess the performances of the tilted-…ber-Bragg- grating-based biosensors. The implementation of a fast measurement technique has contributed to improve the performances of the detection process for low concentrations of target molecules in solution (10 -5 voire 10 -6 g/L). Therefore, the early results seem promising and could provide a conceptual basis for future research: it hbeen demonstrated that a label-free biosensor based on a tilted …ber Bragg grating is able to follow, in real 2. Time, biological interactions taking place at the surface of the sensitive bio…lm. Therefore, this study provides a base for future work regarding, for instance, water pollution monitoring.
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Séverine Maguis. Biocapteurs à fibres optiques fondés sur les réseaux de Bragg en angle fonctionnalisés. Instrumentations et Détecteurs [physics.ins-det]. Ecole Centrale Lyon, 2008. Français. ⟨NNT : 2008ECDL0031⟩. ⟨tel-02271294⟩

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