Polarity Reversal by Centrosome Repositioning Primes Cell Scattering during Epithelial-to-Mesenchymal Transition

Abstract : During epithelial-to-mesenchymal transition (EMT), cells lining the tissue periphery break up their cohesion to migrate within the tissue. This dramatic reorganization involves a poorly characterized reorientation of the apicobasal polarity of static epithelial cells into the front-rear polarity of migrating mesenchymal cells. To investigate the spatial coordination of intracellular reorganization with morphological changes, we monitored centrosome positioning during EMT in vivo, in developing mouse embryos and mammary gland, and in vitro, in cultured 3D cell aggregates and micropatterned cell doublets. In all conditions, centrosomes moved from their off-centered position next to intercellular junctions toward extracellular matrix adhesions on the opposite side of the nucleus, resulting in an effective internal polarity reversal. This move appeared to be supported by controlled microtubule network disassembly. Sequential release of cell confinement using dynamic micropatterns, and modulation of microtubule dynamics, confirmed that centrosome repositioning was responsible for further cell disengagement and scattering.
Document type :
Journal articles
Complete list of metadatas

Cited literature [92 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-02353821
Contributor : Odile Filhol <>
Submitted on : Thursday, November 7, 2019 - 2:36:38 PM
Last modification on : Saturday, November 9, 2019 - 7:48:02 PM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

Citation

Mithila Burute, Magali Prioux, Guillaume Blin, Sandrine Truchet, Gaëlle Letort, et al.. Polarity Reversal by Centrosome Repositioning Primes Cell Scattering during Epithelial-to-Mesenchymal Transition. Developmental Cell, Elsevier, 2017, 40 (2), pp.168-184. ⟨10.1016/j.devcel.2016.12.004⟩. ⟨inserm-02353821⟩

Share

Metrics

Record views

106