De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals - CEA - Commissariat à l’énergie atomique et aux énergies alternatives Access content directly
Journal Articles Nature Communications Year : 2022

De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals

Guillaume Tetreau
  • Function : Author
Michael R. Sawaya
  • Function : Author
Elke De Zitter
  • Function : Author
Anne-Sophie Banneville
  • Function : Author
Natalie A. Schibrowsky
  • Function : Author
Aaron S. Brewster
  • Function : Author
Marie Luise Grünbein
  • Function : Author
Gabriela Nass Kovacs
  • Function : Author
Mark S. Hunter
  • Function : Author
Marco Kloos
  • Function : Author
Raymond G. Sierra
  • Function : Author
Pei Qiao
  • Function : Author
Myriam Stricker
  • Function : Author
Iris D. Young
  • Function : Author
Ninon Zala
  • Function : Author
Alexander Gorel
  • Function : Author
Mario Hilpert
  • Function : Author
Lutz Foucar
  • Function : Author
Johan Bielecki
  • Function : Author
Richard Bean
  • Function : Author
Raphael De Wijn
  • Function : Author
Tokushi Sato
  • Function : Author
Dennis Bideshi
  • Function : Author
Romain Letrun
  • Function : Author
Alexander Batyuk
  • Function : Author
Irina Snigireva
  • Function : Author
Robin Schubert
  • Function : Author
Ethan J. Canfield
  • Function : Author
Mario M. Alba
  • Function : Author
Frédéric Laporte
  • Function : Author
Henry Kirkwood
  • Function : Author
Laurence Després
  • Function : Author
Amandine Roux
  • Function : Author
Christian Chapelle
  • Function : Author
François Riobé
  • Function : Author
Olivier Maury
Sébastien Boutet
  • Function : Author
Adrian Mancuso
  • Function : Author
Thomas R. M. Barends
  • Function : Author
Hyun-Woo Park
  • Function : Author
Arthur D. Laganowsky
  • Function : Author
Jose Rodriguez
  • Function : Author
Manfred Burghammer
  • Function : Author
Robert L. Shoeman
  • Function : Author
R. Bruce Doak
  • Function : Author
Nicholas K. Sauter
  • Function : Author
Brian Federici
  • Function : Author
Duilio Cascio
  • Function : Author
Ilme Schlichting
  • Function : Author

Abstract

Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal Bacillus thuringiensis subsp. israelensis and jegathesan, respectively. The toxins naturally crystallize within the host; however, the crystals are too small for structure determination at synchrotron sources. Therefore, we applied serial femtosecond crystallography at X-ray free electron lasers to in vivo-grown nanocrystals of these toxins. The structure of Cry11Aa was determined de novo using the single-wavelength anomalous dispersion method, which in turn enabled the determination of the Cry11Ba structure by molecular replacement. The two structures reveal a new pattern for in vivo crystallization of Cry toxins, whereby each of their three domains packs with a symmetrically identical domain, and a cleavable crystal packing motif is located within the protoxin rather than at the termini. The diversity of in vivo crystallization patterns suggests explanations for their varied levels of toxicity and rational approaches to improve these toxins for mosquito control.
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Dates and versions

hal-03805376 , version 1 (07-10-2022)

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Guillaume Tetreau, Michael R. Sawaya, Elke De Zitter, Elena A. Andreeva, Anne-Sophie Banneville, et al.. De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals. Nature Communications, 2022, 13, ⟨10.1038/s41467-022-31746-x⟩. ⟨hal-03805376⟩
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