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Production and Characterization of a Novel Exopolysaccharide from Ramlibacter tataouinensis

Desislava Jivkova 1 Ganesan Sathiyanarayanan Mourad Harir Norbert Hertkorn Philippe Schmitt-Kopplin Ghislain Sanhaji Sylvain Fochesato 1 Catherine Berthomieu 2 Alain Heyraud Wafa Achouak 1 Catherine Santaella 1 Thierry Heulin 1 
1 LEMIRE - Laboratoire d'Ecologie Microbienne de la Rhizosphère et d'Environnements Extrêmes
BIAM - Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) : DRF/BIAM
2 IPM - Interactions Protéine Métal
BIAM - Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) : DRF/BIAM
Abstract : The current study examines the desiccation-resistant Ramlibacter tataouinensis TTB310T as a model organism for the production of novel exopolysaccharides and their structural features. This bacterium is able to produce dividing forms of cysts which synthesize cell-bound exopolysaccharide. Initial experiments were conducted on the enrichment of cyst biomass for exopolysaccharide production under batch-fed conditions in a pilot-scale bioreactor, with lactate as the source of carbon and energy. The optimized medium produced significant quantities of exopolysaccharide in a single growth phase, since the production of exopolysaccharide took place during the division of the cysts. The exopolysaccharide layer was extracted from the cysts using a modified trichloroacetic acid method. The biochemical characterization of purified exopolysaccharide was performed by gas chromatography, ultrahigh-resolution mass spectrometry, nuclear magnetic resonance, and Fourier-transform infrared spectrometry. The repeating unit of exopolysaccharide was a decasaccharide consisting of ribose, glucose, rhamnose, galactose, mannose, and glucuronic acid with the ratio 3:2:2:1:1:1, and additional substituents such as acetyl, succinyl, and methyl moieties were also observed as a part of the exopolysaccharide structure. This study contributes to a fundamental understanding of the novel structural features of exopolysaccharide from a dividing form of cysts, and, further, results can be used to study its rheological properties for various industrial applications.
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Submitted on : Saturday, November 19, 2022 - 6:06:07 PM
Last modification on : Sunday, November 20, 2022 - 3:34:12 AM

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Desislava Jivkova, Ganesan Sathiyanarayanan, Mourad Harir, Norbert Hertkorn, Philippe Schmitt-Kopplin, et al.. Production and Characterization of a Novel Exopolysaccharide from Ramlibacter tataouinensis. Molecules, 2022, 27 (21), pp.7172. ⟨10.3390/molecules27217172⟩. ⟨cea-03861333⟩



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