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Journal Articles International Union of Crystallography journal Year : 2022

Serial small- and wide-angle X-ray scattering with laboratory sources

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Abstract

Recent advances in X-ray instrumentation and sample injection systems have enabled serial crystallography of protein nanocrystals and the rapid structural analysis of dynamic processes. However, this progress has been restricted to large-scale X-ray free-electron laser (XFEL) and synchrotron facilities, which are often oversubscribed and have long waiting times. Here, we explore the potential of state-of-the-art laboratory X-ray systems to perform comparable analyses when coupled to micro- and millifluidic sample environments. Our results demonstrate that commercial small- and wide-angle X-ray scattering (SAXS/WAXS) instruments and X-ray diffractometers are ready to access samples and timescales (≳5 ms) relevant to many processes in materials science including the preparation of pharmaceuticals, nanoparticles and functional crystalline materials. Tests of different X-ray instruments highlighted the importance of the optical configuration and revealed that serial WAXS/XRD analysis of the investigated samples was only possible with the higher flux of a microfocus setup. We expect that these results will also stimulate similar developments for structural biology.
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Dates and versions

cea-03757323 , version 1 (22-08-2022)

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Mark A Levenstein, Karen Robertson, Thomas D Turner, Liam Hunter, Cate O'Brien, et al.. Serial small- and wide-angle X-ray scattering with laboratory sources. International Union of Crystallography journal, 2022, 9 (5), ⟨10.1107/S2052252522007631⟩. ⟨cea-03757323⟩
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