A roadmap to high‐resolution standard microcoil MAS NMR spectroscopy for metabolomics

Alan Wong (1)

1 LSDRM - Laboratoire Structure et Dynamique par Résonance Magnétique (LCF)

The current microcoil probe technology has emerged as a significant advancement in NMR applications to biological research of biofluids and continued to excel as a hyphenated tool with other prominent microdevices opening many new possibilities in multiple omics fields. However, this is contrary to biological samples like intact tissue or organism. This is due to the considerable challenges of incorporating the microcoil in a magic-angle spinning (MAS) probe without relinquishing the acquisition of high-resolution spectral data. Not until 2012, a microcoil MAS probe had shown promises on profiling the metabolome in submilligram tissue biopsy with spectral resolution on par with the conventional high-resolution MAS (HR-MAS) NMR. This result had subsequently triggered a great interest in the possibility of NMR analysis with microgram tissues and strived toward the probe development of 'high-resolution' capable microcoil MAS NMR spectroscopy. This review gives an overview of the issues and challenges in the probe development and summarizes the advancements toward metabolomics.

Mots clés: Microcoils; Magic angle spinning MAS; high resolution; Sensitivity Analysis; metabolomics

Domaines: Chimie analytique; Chimie-Physique [physics.chem-ph]

Alan Wong. A roadmap to high‐resolution standard microcoil MAS NMR spectroscopy for metabolomics. NMR in Biomedicine, 2022, pp.e4683. ⟨10.1002/nbm.4683⟩. ⟨cea-03509128⟩