Metabolic NMR mapping with microgram tissue biopsy - Archive ouverte HAL Access content directly
Journal Articles NMR in Biomedicine Year : 2021

Metabolic NMR mapping with microgram tissue biopsy

(1) , (2) , (2) , (2) , (1)
1
2

Abstract

This study explores the potential of profiling a microgram-scale soft tissue biopsy by NMR spectroscopy. The important elements of high resolution and high sensitivity for the spectral data are achieved through a unique probe, HR-μMAS, which allowed comprehensive profiling to be performed on microgram tissue for the first time under MAS conditions. Thorough spatially resolved metabolic maps were acquired across a coronal brain slice of rat C6 gliomas, which rendered the delineation of the tumor lesion. The results present a unique ex vivo NMR possibility to analyze tissue pathology that cannot be fully explored by the conventional approach, HR-MAS and in vivo MRS. Aside from the capability of analyzing a small localized region to track its specific metabolism, it could also offer the possibility to carry out longitudinal investigations on live animals due to the feasibility of minimally invasive tissue excision.
Fichier principal
Vignette du fichier
NMR Biomed 2020.pdf (3.89 Mo) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

cea-03126683 , version 1 (15-10-2021)

Identifiers

Cite

Covadonga Lucas‐torres, Hélène Roumes, Véronique Bouchaud, Anne‐karine Bouzier‐sore, Alan Wong. Metabolic NMR mapping with microgram tissue biopsy. NMR in Biomedicine, 2021, 34 (4), pp.e4477. ⟨10.1002/nbm.4477⟩. ⟨cea-03126683⟩
40 View
76 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More