Quantification of guanosine triphosphate and tetraphosphate in plants and algae using stable isotope-labelled internal standards

Julia Bartoli (1) , Sylvie Citerne (2) , Gregory Mouille (2) , Emmanuelle Bouveret (3) , Ben Field (4)

1 LISM - Laboratoire d'ingénierie des systèmes macromoléculaires
2 IJPB - Institut Jean-Pierre Bourgin
3 Adaptation au stress et métabolisme chez les entérobactéries - Stress adaptation and metabolism in enterobacteria
4 LGBP - Luminy Génétique et Biophysique des Plantes

Guanosine tetraphosphate (G4P) and guanosine pentaphosphate (G5P) are signalling nucleotides found in bacteria and photosynthetic eukaryotes that are implicated in a wide-range of processes including stress acclimation, developmental transitions and growth control. Measurements of G4P/G5P levels are essential for studying the diverse roles of these nucleotides. However, G4P/G5P quantification is particularly challenging in plants and algae due to lower cellular concentrations, compartmentalization and high metabolic complexity. Despite recent advances the speed and accuracy of G4P quantification in plants and algae can still be improved. Here, we report a new approach for rapid and accurate G4P quantification which relies on the use of synthesized stable isotope-labelled as internal standards. We anticipate that this approach will accelerate research into the function of G4P signaling in plants, algae and other organisms.

Keywords: Guanosine tetraphosphate; Bacteria; Plants; Algae; Stable isotope labelling; LC-MS/MS

Domains: Life Sciences [q-bio]; Life Sciences [q-bio]; Vegetal Biology; Life Sciences [q-bio]; Microbiology and Parasitology; Bacteriology

Julia Bartoli, Sylvie Citerne, Gregory Mouille, Emmanuelle Bouveret, Ben Field. Quantification of guanosine triphosphate and tetraphosphate in plants and algae using stable isotope-labelled internal standards. Talanta, 2020, 219, pp.121261. ⟨10.1016/j.talanta.2020.121261⟩. ⟨cea-02961710⟩