Skip to Main content Skip to Navigation
Journal articles

Toxicity to RAW264.7 Macrophages of Silica Nanoparticles and the E551 Food Additive, in Combination with Genotoxic Agents

Fanny Dussert 1, 2 Pierre-Adrien Arthaud 1, 2 Marie-Edith Arnal 1, 2 Bastien Dalzon 3 Anaëlle Torres 3 Thierry Douki 1, 2 Nathalie Herlin 4 Thierry Rabilloud 3 Marie Carrière 1, 2, *
* Corresponding author
1 CIBEST [2020-....] - Chimie Interface Biologie pour l’Environnement, la Santé et la Toxicologie [2020-....]
SYMMES - SYstèmes Moléculaires et nanoMatériaux pour l’Energie et la Santé : DRF/IRIG/SYMMES
3 ProMD [2020-....] - Protéomique, Métaux et Différenciation [2020-....]
LCBM - UMR 5249 - Laboratoire de Chimie et Biologie des Métaux
4 LEDNA - Laboratoire Edifices Nanométriques
NIMBE UMR 3685 - Nanosciences et Innovation pour les Matériaux, la Biomédecine et l'Energie (ex SIS2M)
Abstract : Synthetic amorphous silica (SAS) is used in a plethora of applications and included in many daily products to which humans are exposed via inhalation, ingestion, or skin contact. This poses the question of their potential toxicity, particularly towards macrophages, which show specific sensitivity to this material. SAS represents an ideal candidate for the adsorption of environmental contaminants due to its large surface area and could consequently modulate their toxicity. In this study, we assessed the toxicity towards macrophages and intestinal epithelial cells of three SAS particles, either isolated SiO 2 nanoparticles (LS30) or SiO 2 particles composed of agglomerated-aggregates of fused primary particles, either food-grade (E551) or non-food-grade (Fumed silica). These particles were applied to cells either alone or in combination with genotoxic co-contaminants, i.e., benzo[a]pyrene (B[a]P) and methane methylsulfonate (MMS). We show that macrophages are much more sensitive to these toxic agents than a non-differenciated co-culture of Caco-2 and HT29-MTX cells, used here as a model of intestinal epithelium. Co-exposure to SiO 2 and MMS causes DNA damage in a synergistic way, which is not explained by the modulation of DNA repair protein mRNA expression. Together, this suggests that SiO 2 particles could adsorb genotoxic agents on their surface and, consequently, increase their DNA damaging potential.
Document type :
Journal articles
Complete list of metadatas

Cited literature [51 references]  Display  Hide  Download

https://hal-cea.archives-ouvertes.fr/cea-02907951
Contributor : Serge Palacin <>
Submitted on : Tuesday, July 28, 2020 - 8:35:15 AM
Last modification on : Wednesday, October 14, 2020 - 4:20:54 AM

File

nanomaterials-10-01418-v2.pdf
Publisher files allowed on an open archive

Identifiers

Citation

Fanny Dussert, Pierre-Adrien Arthaud, Marie-Edith Arnal, Bastien Dalzon, Anaëlle Torres, et al.. Toxicity to RAW264.7 Macrophages of Silica Nanoparticles and the E551 Food Additive, in Combination with Genotoxic Agents. Nanomaterials, MDPI, 2020, 10 (7), pp.1418. ⟨10.3390/nano10071418⟩. ⟨cea-02907951⟩

Share

Metrics

Record views

101

Files downloads

75