Disassembly of Tau fibrils by the human Hsp70 disaggregation machinery generates small seeding-competent species - Archive ouverte HAL Access content directly
Journal Articles Journal of Biological Chemistry Year : 2020

Disassembly of Tau fibrils by the human Hsp70 disaggregation machinery generates small seeding-competent species

(1, 2) , (1) , (3) , (3) , (4) , (5) , (6) , (4) , (2) , (3) , (1) , (1) , (1)
1
2
3
4
5
6
Harm H. Kampinga
  • Function : Author
  • PersonId : 1063846

Abstract

The accumulation of amyloid Tau aggregates is implicated in Alzheimer’s disease (AD) and other tauopathies. Molecular chaperones are known to maintain protein homeostasis. Here we show that an ATP-dependent human chaperone system disassembles Tau fibrils in vitro. We found that this function is mediated by the core chaperone HSC70, assisted by specific co-chaperones, in particular class B J-domain proteins and a heat shock protein 110 (Hsp110)-type nucleotide exchange factor (NEF). The Hsp70 disaggregation machinery processed recombinant fibrils assembled from all six Tau isoforms as well as sarkosylresistant Tau aggregates extracted from cell cultures and human AD brain tissues, demonstrating the ability of the Hsp70 machinery to recognize a broad range of Tau aggregates. However, the chaperone activity released monomeric and small oligomeric Tau species, which induced the aggregation of selfpropagating Tau conformers in a Tau cell culture model. We conclude that the activity of the Hsp70 disaggregation machinery is a double-sided sword, as it eliminates Tau amyloids at the cost of generating new seeds.
Fichier principal
Vignette du fichier
2020_Tau disaggregation_Hsp70_Melki_Nussbaum-Krammer_JBiolChem.pdf (5.92 Mo) Télécharger le fichier
Origin : Files produced by the author(s)
Loading...

Dates and versions

cea-02733373 , version 1 (02-06-2020)

Identifiers

Cite

Eliana Nachman, Anne Wentink, Karine Madiona, Luc Bousset, Taxiarchis Katsinelos, et al.. Disassembly of Tau fibrils by the human Hsp70 disaggregation machinery generates small seeding-competent species. Journal of Biological Chemistry, 2020, pp.jbc.RA120.013478. ⟨10.1074/jbc.RA120.013478⟩. ⟨cea-02733373⟩
115 View
78 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More