Glucose availability dictates the export of the soluble and prion forms of Sup35p via periplasmic or extracellular vesicles - Archive ouverte HAL Access content directly
Journal Articles Molecular Microbiology Year : 2020

Glucose availability dictates the export of the soluble and prion forms of Sup35p via periplasmic or extracellular vesicles

(1) , (1) , (1)
1

Abstract

The yeast [PSI$^+$] prion originates from the self‐perpetuating transmissible aggregates of the translation termination factor Sup35p. We previously showed that infectious Sup35p particles are exported outside the cells via extracellular vesicles (EV). This finding suggested a function for EV in the vertical and horizontal transmission of yeast prions. Here we report a significant export of Sup35p within periplasmic vesicles (PV) upon glucose starvation. We show that PV are up to three orders of magnitude more abundant than EV. However, PV and EV are different in terms of size and protein content, and their export is oppositely regulated by glucose availability in the growth medium. Overall, our work suggests that the export of prion particles to both the periplasm and the extracellular space needs to be considered to address the physiological consequences of vesicle‐mediated yeast prions trafficking.
Fichier principal
Vignette du fichier
mmi.14515 (accepted version online_27042020).pdf (2.76 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Loading...

Dates and versions

cea-02556526 , version 1 (05-11-2020)

Identifiers

Cite

Mehdi Kabani, Marion Pilard, Ronald Melki. Glucose availability dictates the export of the soluble and prion forms of Sup35p via periplasmic or extracellular vesicles. Molecular Microbiology, 2020, 114, pp.322 - 332. ⟨10.1111/mmi.14515⟩. ⟨cea-02556526⟩
47 View
54 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More