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Facile and fast detection of bacteria via the detection of exogenous volatile metabolites released by enzymatic hydrolysis

Laure-Hélène Guillemot 1, 2 Marjorie Vrignaud 3 Pierre Marcoux 1 Charles Rivron 4 Thu-Hoa Tran-Thi 2, 4
3 SBSC - Service Bio-System on Chip
DTBS - Département Microtechnologies pour la Biologie et la Santé : DRT/LETI/DTBS
4 LEDNA - Laboratoire Edifices Nanométriques
NIMBE UMR 3685 - Nanosciences et Innovation pour les Matériaux, la Biomédecine et l'Energie (ex SIS2M)
Abstract : A low-cost, innovative and non-invasive colorimetric test, which can be universally used, is proposed to detect pathogen bacteria via the simple and fast detection of volatile metabolites released by enzymatic hydrolysis. The proof of 10 concept is shown via three sets of experiments studying the release of the p-nitrophenol metabolite in solution in the E. coli cultures containing 4-nitrophenyl-β-D-glucuronide, the trapping efficiency of the gaseous metabolite by various tailored and functionalized xerogels, and the trapping and 15 detection of gaseous p-nitrophenol released by E. coli bacteria.
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Submitted on : Wednesday, April 1, 2020 - 6:51:23 PM
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Laure-Hélène Guillemot, Marjorie Vrignaud, Pierre Marcoux, Charles Rivron, Thu-Hoa Tran-Thi. Facile and fast detection of bacteria via the detection of exogenous volatile metabolites released by enzymatic hydrolysis. Physical Chemistry Chemical Physics, Royal Society of Chemistry, 2013, 15 (38), pp.15840. ⟨10.1039/c3cp51281a⟩. ⟨cea-02528474⟩

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