Facile and fast detection of bacteria via the detection of exogenous volatile metabolites released by enzymatic hydrolysis

Laure-Hélène Guillemot (1, 2) , Marjorie Vrignaud (3) , Pierre R. Marcoux (1) , Charles Rivron (4) , Thu-Hoa Tran-Thi (2, 4)

1 DTBS - Département Microtechnologies pour la Biologie et la Santé
2 LFP - URA 2453 - Laboratoire Francis PERRIN
3 SBSC - Service Bio-System on Chip
4 LEDNA - Laboratoire Edifices Nanométriques

A low-cost, innovative and non-invasive colorimetric test, which can be universally used, is proposed to detect pathogen bacteria via the simple and fast detection of volatile metabolites released by enzymatic hydrolysis. The proof of 10 concept is shown via three sets of experiments studying the release of the p-nitrophenol metabolite in solution in the E. coli cultures containing 4-nitrophenyl-β-D-glucuronide, the trapping efficiency of the gaseous metabolite by various tailored and functionalized xerogels, and the trapping and 15 detection of gaseous p-nitrophenol released by E. coli bacteria.

Keywords: colorimetric detection; p-nitrophenol; microbial volatile organic compound; E. coli; Xerogel; Sol-gel; bacteria detection

Domains: Chemical Sciences; Material chemistry; Chemical Sciences; Inorganic chemistry; Life Sciences [q-bio]; Microbiology and Parasitology; Bacteriology

Laure-Hélène Guillemot, Marjorie Vrignaud, Pierre R. Marcoux, Charles Rivron, Thu-Hoa Tran-Thi. Facile and fast detection of bacteria via the detection of exogenous volatile metabolites released by enzymatic hydrolysis. Physical Chemistry Chemical Physics, 2013, 15 (38), pp.15840. ⟨10.1039/c3cp51281a⟩. ⟨cea-02528474⟩