General Guidelines for Sample Preparation Strategies in HR-$\mu$MAS NMR-based Metabolomics of Microscopic Specimens - Archive ouverte HAL Access content directly
Journal Articles Metabolites Year : 2020

General Guidelines for Sample Preparation Strategies in HR-$\mu$MAS NMR-based Metabolomics of Microscopic Specimens

(1) , (2) , (1) , (1) , (3) , (4) , (4) , (5) , (5) , (6) , (1)
1
2
3
4
5
6
Yusuke Nishiyama

Abstract

The study of the metabolome within tissues, organisms, cells or biofluids can be carried out by several bioanalytical techniques. Among them, nuclear magnetic resonance (NMR) is one of the principal spectroscopic methods. This is due to a sample rotation technique, high-resolution magic angle spinning (HR-MAS), which targets the analysis of heterogeneous specimens with a bulk sample mass from 5 to 10 mg. Recently, a new approach, high-resolution micro-magic angle spinning (HR-$\mu$MAS), has been introduced. It opens, for the first time, the possibility of investigating microscopic specimens (<500 $\mu$g) with NMR spectroscopy, strengthening the concept of homogeneous sampling in a heterogeneous specimen. As in all bioanalytical approaches, a clean and reliable sample preparation strategy is a significant component in designing metabolomics (oromics, in general) studies. The sample preparation for HR-$\mu$MAS is consequentially complicated by the $\mu$g-scale specimen and has yet to be addressed. This report details the strategies for three specimen types: biofluids, fluid matrices and tissues. It also provides the basis for designing future μMAS NMR studies of microscopic specimens.
Fichier principal
Vignette du fichier
metabolites-10-00054.pdf (1.11 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Loading...

Dates and versions

cea-02464528 , version 1 (03-02-2020)

Identifiers

Cite

Covadonga Lucas-Torres, Thierry Bernard, Gaspard Huber, Patrick Berthault, Yusuke Nishiyama, et al.. General Guidelines for Sample Preparation Strategies in HR-$\mu$MAS NMR-based Metabolomics of Microscopic Specimens. Metabolites, 2020, 10 (2), pp.54. ⟨10.3390/metabo10020054⟩. ⟨cea-02464528⟩
49 View
74 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More