Skip to Main content Skip to Navigation
Journal articles

Identification of the periplasmic DNA receptor for natural transformation of Helicobacter pylori

Abstract : Horizontal gene transfer through natural transformation is a major driver of antibiotic resistance spreading in many pathogenic bacterial species. In the case of Gram-negative bacteria, and in particular of Helicobacter pylori, the mechanisms underlying the handling of the incoming DNA within the periplasm are poorly understood. Here we identify the protein ComH as the periplasmic receptor for the transforming DNA during natural transformation in H. pylori. ComH is a DNA-binding protein required for the import of DNA into the periplasm. Its C-terminal domain displays strong affinity for double-stranded DNA and is sufficient for the accumulation of DNA in the periplasm, but not for DNA internalisation into the cytoplasm. The N-terminal region of the protein allows the interaction of ComH with a periplasmic domain of the inner-membrane channel ComEC, which is known to mediate the translocation of DNA into the cytoplasm. Our results indicate that ComH is involved in the import of DNA into the periplasm and its delivery to the inner membrane translocator ComEC.
Complete list of metadatas

https://hal-cea.archives-ouvertes.fr/cea-02386037
Contributor : J. Pablo Radicella <>
Submitted on : Friday, November 29, 2019 - 10:34:46 AM
Last modification on : Friday, September 11, 2020 - 3:18:04 AM

Links full text

Identifiers

Citation

Prashant Damke, Anne Marie Di Guilmi, Paloma Fernandez Varela, Christophe Velours, Stéphanie Marsin, et al.. Identification of the periplasmic DNA receptor for natural transformation of Helicobacter pylori. Nature Communications, Nature Publishing Group, 2019, 10 (1), pp.5357. ⟨10.1038/s41467-019-13352-6⟩. ⟨cea-02386037⟩

Share

Metrics

Record views

109