A doubly responsive probe for the detection of Cys4-tagged proteins

Emilie Mari (1) , Catherine Boutin (1) , E. Léonce (1) , B Rousseau (2) , Marie Erard (3) , P. Berthault (1)

1 LSDRM - Laboratoire Structure et Dynamique par Résonance Magnétique (LCF)
2 SCBM - Service de Chimie Bio-Organique et de Marquage
3 LCPO - Laboratoire de Chimie Physique D'Orsay

Introduction Full understanding of intracellular phenomena involves sensitive and non-invasive detection. A less disruptive method than labeling of fluorescent proteins uses binding between a tag of only six natural amine acids that can be genetically incorporated into the protein of interest and a small molecule ca lied FIAsH[1]. This molecule has the ability to fluoresce only when it binds to its 4Cys-tag target. Another technique based on 129Xe NMR has emerged. Xenon is hyperpolarized to enhance the NMR signal by orders of magnitude and its reversible encapsulation in functionalized host systems gives it a specifie spectral signature[2]. Capability of the noble gas to cross cell membranes without losing its polarization[3] enables in cellule investigations. Here we report the first design and study of a dual fluorescence-and 129Xe NMR-based sensor of Cys4-tagged proteins[4].

Domains: Material chemistry

Emilie Mari, Catherine Boutin, E. Léonce, B Rousseau, Marie Erard, et al.. A doubly responsive probe for the detection of Cys4-tagged proteins. European Molecular Imaging Meeting, Mar 2016, Utrecht, Netherlands. ⟨cea-02346358⟩