Ultra-sensitive detection method with droplet based microfluidic device coupled to MALDI-TOF

Kiarach Mesbah (1) , Sarah Bregant (2) , Florent Malloggi (1)

1 LIONS - Laboratoire Interdisciplinaire sur l'Organisation Nanométrique et Supramoléculaire
2 SIMoS - Service d'Ingénierie Moléculaire pour la Santé (ex SIMOPRO)

We present an automated microfluidic droplet generator/depositor for MALDI-TOF analysis allowing attomol detection of peptides from sub-nanomolar soltutions: a sensitivity which is a paramount importance for proteomics and diagnosis field. Droplet based microfluidic devices offer many advantages in integration of multiple biological or chemical processes, essential tools for biomarker/biological fluids analysis [1]. However the detection mainly relies on optical methods such as fluorescence which implies a labelling of target molecules but also suffers from a lack of sensitivity. At the opposite mass spectrometry is a highly sensitive technic based on a label free detection of analytes but interfacing droplet-based microfluidic and mass spectrometry is challenging and up to now there are few studies reported [2-3]. We focus on the integration of a droplet based microfluidic system with a MALDI-TOF. We develop methods which prevent the use of surfactants: a crippling factor since surfactants led to high background noise, and then are not compatible with high sensitivity detection method. To circumvent this effect, we designed a chip which prevents droplets coalescence by spacing each droplet with a controlled volume of oil (Figure 1). This simple geometry allows to finely tune the amount of spotted droplets and controlled the coalescence. The microfluidic chip outlet is then connected to a capillary, and the droplets are transferred to a MALDI plate mounted on a motorized xy-stage. To estimate the potential of the platform, we developed a full optimized method (sample composition, matrix composition, droplet generation, deposition and analysis) of relevant proteomics biomarker: a native peptide Angiotensin II involved in several diseases [4-5]. First, we compared our system with a standard MALDI deposition procedure i.e. manual pipetting. The spectra of Figure 2 show 500nl of a solution of 10fmol/µl Angiotensin II-left pipette deposited and-right platform deposited. In both cases, the 1046.2 m/z peak of Angiotensin II is visible but in the former case the intensity is increased by one order of magnitude. A closer look to the deposits shows a concentration effect with the platform deposition (see insets figure 2). In a second step, we made a sensitivity test by depositing different concentrations of analytes. The Figure 3 shows the average peak intensity as a function of n the quantity deposited in femtomole. We record good intensity signal down to 100 attomoles (see inset figure 3). Below this threshold the spectra are very noisy (Figure 4-left). In order to increase the sensitivity of the method, we developed a multi-spotting deposit. By repeated deposition of droplets, we increase drastically the amount of peptides on the spot, and can then reach very low level of detection. As a proof the Figure 4 shows the spectra of sub-nM solution before (left) and after the multideposit (right). We developed a method which allows to generate single droplet in a microchip coupled with a MALDI-TOF analysis. We control the volume of sample deposit on each spot, and by multi-spotting we reached a very high sensitivity compatible with physiological concentration of proteomic biomarkers.

Domaines: Matériaux

Kiarach Mesbah, Sarah Bregant, Florent Malloggi. Ultra-sensitive detection method with droplet based microfluidic device coupled to MALDI-TOF. MicroTAS, Oct 2016, Dublin, Ireland. ⟨cea-02346351⟩