Introducing HR-$\mu$MAS NMR probe for $\mu$g-scale samples
Résumé
NMR has already proven to be a tremendqus spectroscopic tool in metabolomics. However, due to its low detection sensitivity, NMR analyses can be challenging especially for mass-limited samples. To overcome this issue, here we introduce a 'new' reliable methodology for profiling $\mu$g/nL scale samples based on the Magic Angle Spinning (MAS) technique-High-Resolution lIMAS (HR-$\mu$MAS). HR-$\mu$MAS offers an outstanding opportunity for the detection of metabolites in $\mu$g samples with good sensitivity and high spectral resolution, which coutd widen the existing NMR-based spectroscopy to metabolomic applications and pave the path to its utilization in numerous fields of study. ln comparison to the previous approach with the spinning $\mu$-coil (HR-MACS) [1], HR-$\mu$MAS renders improvements in probe practicality, sample-preparation, data repeatability and in the flexibility of NMR experiments. Moreover, we also show the possibility of performing metabolic quantification using HR-$\mu$ MAS-important analytical component in metabolomics. The excellent NMR spectroscopic capabilities of HR-$\mu$MAS shown here for NMR metabolic profiling on $\mu$g/nL samples have demonstrated that HR-$\mu$MAS could have an impact on future NMR-based metabolomics in biological applications.
Domaines
Matériaux
Origine : Fichiers produits par l'(les) auteur(s)