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The development of HR-MAS NMR towards microgramm biospecimens

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Abstract

NMR has already proven to be a tremendous spectroscopic Lool in the field of metabolomics in living specimens. Its major weakness is the low detection sensitivity that renders the analysis of microscopic quantities $<$1mg-scale) impractical, time consuming and often impossible. The use of micro-size NMR detection coils is consider a cost effective approach; however, implementing a microcoil for heterogeneous biospecimens su ch as tissues, cells and organisms is a challenging task. This is because of the necessity of rapid sample rotation of the specimens at a specific angle, 54.74$\circ$, to the magnetic field. The technique den otes as Magic-Angle Spinning (MAS) NMR. It is comlllonly applied to solid materials. Depending on the diameter of the MAS detection coil, MAS can be applied to a wide range of sample mass h·om 500 mg with a large coil diameter 7-mm probe, to < Img with $\mu$-size diameter MAS probe (1-mm and 0.7-mm). However, these $\mu$MAS probes are not applicable to metabolomic studies due to the inadequate spectral resolution (0.02 ppm). For this reason, there are no suit able $\mu$MAS probes for metabolomic studies prior to 2014. This talk will present the 'progress' of the MAS developments towards metabolomics of $\mu$g specimens. It will briefly outline (i.e. sensitivity, resolution and practicality) two different $\mu$MAS approaches: (i) using a $\mu$-size inductively coupled resonator, High-Resolution Magic Angle Coil Spinning (HRMACS); and (ii) the use of a specially designed standalone $\mu$MAS probe, High-Resolution micro-Magic Angle Spinning (HR$\mu$MAS).
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Dates and versions

cea-02340805 , version 1 (31-10-2019)

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  • HAL Id : cea-02340805 , version 1

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Alan Wong, Yusuke Nishiyama. The development of HR-MAS NMR towards microgramm biospecimens. SMMAP 2017, Oct 2017, Marne-la-Vallée (Disneyland Paris), France. ⟨cea-02340805⟩
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