Skip to Main content Skip to Navigation
Conference papers

HR-$\mu$MAS NMR localized metabolic profiling of $\mu$g samples: a model study

Covadonga Lucas-Torres 1, * Yusuke Nishiyama 2 Alan Wong 1
* Corresponding author
1 LSDRM - Laboratoire Structure et Dynamique par Résonance Magnétique (LCF)
NIMBE UMR 3685 - Nanosciences et Innovation pour les Matériaux, la Biomédecine et l'Energie (ex SIS2M)
Abstract : NMR has already proven to be a tremendous spectroscopic tool in metabonomics. However, due to its low detection sensitivity, NMR analyses can be challenging especially for mass-limited samples. To overcome this issue, here we introduce a 'new' reliable methodology for profiling $\mu$g/nL scale samples based on the Magic Angle Spinning (MAS) technique-High-Resolution $\mu$MAS (i.e. 1 mm HR-$\mu$MAS). A model study has been proposed: the localized metabolic profiling (i. e. mapping) of a garlic clove. We have been able to investigate 4 different regions (clove skin, flesh, core and inner epidermis), whereas only 3 regions were distinguished using the standard approach, HR-MAS with larger sample mass ($\sim$10 mg). The use of multivariate statistical analysis allowed an initial separation between the different regions by unsupervised PCA. Based on this PCA, a supervised discriminant analysis OPLS was carried out between the previously separated clusters to find the discriminant metabolites. The fact that these micro-detections have been able to offer such precise and accurate results on a model study, opens the way to new applications on heterogeneous biospecimens (such as tissues and cells), on the determination of NMR-based localized metabolic profiles to get the comprehensive fingerprint of their different anatomical regions.
Document type :
Conference papers
Complete list of metadatas

Cited literature [2 references]  Display  Hide  Download

https://hal-cea.archives-ouvertes.fr/cea-02327970
Contributor : Serge Palacin <>
Submitted on : Wednesday, October 23, 2019 - 9:23:54 AM
Last modification on : Monday, February 10, 2020 - 6:12:49 PM
Long-term archiving on: : Friday, January 24, 2020 - 2:47:49 PM

File

LucasTorres_June2018a.pdf
Files produced by the author(s)

Identifiers

  • HAL Id : cea-02327970, version 1

Citation

Covadonga Lucas-Torres, Yusuke Nishiyama, Alan Wong. HR-$\mu$MAS NMR localized metabolic profiling of $\mu$g samples: a model study. VIII Ibero-American NMR meeting # 6th Iberian NMR Meeting # 9th GERMN # 4th Portuguese RNRMN Meeting, Jun 2018, Lisbon, Portugal. ⟨cea-02327970⟩

Share

Metrics

Record views

28

Files downloads

21