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Stable Isotope Probing of Microbiota Structure and Function in the Plant Rhizosphere
Abstract : Stable isotope probing of microbial nucleic acids applied in the rhizosphere enables (a) the identification of the active microbial community involved in root exudate assimilation and those involved in soil organic matter degradation, and (b) the study of the impact of plants via root exudates on the in situ expression of microbial functions. By incubating plants under (CO2)-C-13, fresh carbon exuded by the plant will be labeled and hence the microbial community assimilating C-13-root exudates will incorporate C-13 into their cellular macromolecules. Labeled DNA, RNA, and proteins can be used to identify microorganisms that assimilated the root exudates. We provide a step-by-step protocol on how to apply stable isotope probing of DNA and RNA in the plant rhizosphere to identify the active microbial communities and analyze their gene expression.
https://hal-cea.archives-ouvertes.fr/cea-02327672
Contributor : Alexandra Maraval Connect in order to contact the contributor
Submitted on : Tuesday, October 22, 2019 - 10:20:44 PM
Last modification on : Friday, January 7, 2022 - 12:46:02 PM
Contributor : Alexandra Maraval Connect in order to contact the contributor
Submitted on : Tuesday, October 22, 2019 - 10:20:44 PM
Last modification on : Friday, January 7, 2022 - 12:46:02 PM