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Journal articles
XNA ligation using T4 DNA ligase in crowding conditions
Donaat Kestemont
1
Marleen Renders
1
Piotr Leonczak
1
Mikhail Abramov
1
Guy Schepers
1
Vitor B. Pinheiro
2, 3
Jef Rozenski
1
Piet Herdewijn
1, 4
Guy Schepers 1
Author
Vitor B. Pinheiro 2, 3
Author
Jef Rozenski 1
Author
Piet Herdewijn 1, 4
Author
1
Rega Institute for Medical Research [Leuven, België] (Minderbroedersstraat 10, B 3000 Leuven - Belgium)
- KU Leuven - Catholic University of Leuven - Katholieke Universiteit Leuven (Oude Markt 13 - bus 5005, 3000 Leuven - Belgium)
2
Birkbeck College [University of London] (Malet St, London WC1E 7HX, Royaume-Uni - United Kingdom)
3
UCL - University College of London [London] (Gower Street, London WC1E 6BT - United Kingdom)
4
UMR 8030 - Génomique métabolique (GENOSCOPE, 2 rue Gaston Crémieux 91057 Evry Cedex - France)
- GENOSCOPE - Genoscope - Centre national de séquençage [Evry] : DRF/JACOB (2, rue Gaston Crémieux CP5706 91057 EVRY Cedex - France)
- Université Paris-Saclay (Espace Technologique, Bat. Discovery - RD 128 - 2e ét., 91190 Saint-Aubin - France)
- DRF (CEA) - Direction de Recherche Fondamentale (CEA) : DRF/JACOB (Direction de Recherche Fondamentale
Fundamental Research Division
CEA Saclay
91191 Gif-sur-Yvette
- France)
- CEA - Commissariat à l'énergie atomique et aux énergies alternatives (Centre de Saclay Centre de Grenoble Centre de Cadarache etc - France)
- CNRS - Centre National de la Recherche Scientifique : UMR8030 (France)
- UEVE - Université d'Évry-Val-d'Essonne (Boulevard François Mitterrand 91025 Evry Cedex - France)
Abstract : T4 DNA ligase is capable of ligating 2OMe-RNA duplexes, HNA, LNA and FANA mixed sequences in the presence of 10% w/v PEG8000 and 3 M betaine. The enzymatic joining of oligonucleotides containing multiple consecutive XNA nucleotides at the ligation site has not been reported before.
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https://hal-cea.archives-ouvertes.fr/cea-02291354
Contributor : Marianne Leriche <>
Submitted on : Wednesday, September 18, 2019 - 4:21:15 PM
Last modification on : Wednesday, June 17, 2020 - 3:42:03 PM
Contributor : Marianne Leriche <>
Submitted on : Wednesday, September 18, 2019 - 4:21:15 PM
Last modification on : Wednesday, June 17, 2020 - 3:42:03 PM
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- HAL Id : cea-02291354, version 1
- DOI : 10.1039/c8cc02414f
- PUBMED : 29872779
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Citation
Donaat Kestemont, Marleen Renders, Piotr Leonczak, Mikhail Abramov, Guy Schepers, et al.. XNA ligation using T4 DNA ligase in crowding conditions. Chemical Communications, Royal Society of Chemistry, 2018, 54 (49), pp.6408-6411. ⟨10.1039/c8cc02414f⟩. ⟨cea-02291354⟩
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