XNA ligation using T4 DNA ligase in crowding conditions

Donaat Kestemont (1) , Marleen Renders (1) , Piotr Leonczak (1) , Mikhail Abramov (1) , Guy Schepers (1) , Vitor B. Pinheiro (2, 3) , Jef Rozenski (1) , Piet Herdewijn (1, 4)

1 Rega Institute for Medical Research [Leuven, België]
2 Birkbeck College [University of London]
3 UCL - University College of London [London]
4 UMR 8030 - Génomique métabolique

T4 DNA ligase is capable of ligating 2OMe-RNA duplexes, HNA, LNA and FANA mixed sequences in the presence of 10% w/v PEG8000 and 3 M betaine. The enzymatic joining of oligonucleotides containing multiple consecutive XNA nucleotides at the ligation site has not been reported before.

Domains: Life Sciences [q-bio]

Donaat Kestemont, Marleen Renders, Piotr Leonczak, Mikhail Abramov, Guy Schepers, et al.. XNA ligation using T4 DNA ligase in crowding conditions. Chemical Communications, 2018, 54 (49), pp.6408-6411. ⟨10.1039/c8cc02414f⟩. ⟨cea-02291354⟩