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Journal Articles Chemical Communications Year : 2018

XNA ligation using T4 DNA ligase in crowding conditions

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Abstract

T4 DNA ligase is capable of ligating 2OMe-RNA duplexes, HNA, LNA and FANA mixed sequences in the presence of 10% w/v PEG8000 and 3 M betaine. The enzymatic joining of oligonucleotides containing multiple consecutive XNA nucleotides at the ligation site has not been reported before.

Dates and versions

cea-02291354 , version 1 (18-09-2019)

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Donaat Kestemont, Marleen Renders, Piotr Leonczak, Mikhail Abramov, Guy Schepers, et al.. XNA ligation using T4 DNA ligase in crowding conditions. Chemical Communications, 2018, 54 (49), pp.6408-6411. ⟨10.1039/c8cc02414f⟩. ⟨cea-02291354⟩
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