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USA) equipped with a clamp-on leaf cuvette (6400-40 Leaf Chamber Fluorometer ,
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, For measuring the turgid weight (TW), samples were submerged with bi-distilled water and left at 4°C for 16 hours
Six leaves per condition were cut from ?-CCA-treated or -untreated plants and weighted (FW), and placed in 25 ml of bi-distilled water. Conductivity of the and Psypro control unit (Wescor, 1992. ,
, Total RNA was isolated from 100 mg leaves using the Nucleospin® RNA Plant kit (Macherey-Nagel). The concentration was measured on a NanoDrop2000, RNA isolation and qRT-PCR
, 10 µM each of forward and reverse primers and water, was added to 2 µL of a 10-fold diluted cDNA sample in a 384 well plate, Lightcycler 480 Real-Time PCR system
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10 mM Mes/Tris, pH 6). Stomata were analyzed by using an inverted microscope (Leica LMD-6000) ,
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