Analytical ultracentrifugation for analysis of doxorubicin loaded liposomes - CEA - Commissariat à l’énergie atomique et aux énergies alternatives Accéder directement au contenu
Article Dans Une Revue International Journal of Pharmaceutics Année : 2017

Analytical ultracentrifugation for analysis of doxorubicin loaded liposomes

Résumé

Analytical ultracentrifugation (AUC) is a powerful tool for the study of particle size distributions and interactions with high accuracy and resolution. In this work, we show how the analysis of sedimentation velocity data from the AUC can be used to characterize nanocarrier drug delivery systems used in nanomedicine. Nanocarrier size distribution and the ratio of free versus nanoparticle-encapsulated drug in a commercially available liposomal doxorubicin formulation are determined using interference and absorbance based AUC measurements and compared with results generated with conventional techniques. Additionally, the potential of AUC in measuring particle density and the detection of nanocarrier sub-populations is discussed as well. The unique capability of AUC in providing reliable data for size and composition in a single measurement and without complex sample preparation makes this characterization technique a promising tool both in nanomedicine product development and quality control.

Dates et versions

cea-02202414 , version 1 (31-07-2019)

Identifiants

Citer

Dora Mehn, Patrizia Iavicoli, Noelia Cabaleiro, Sven Even Borgos, Fanny Caputo, et al.. Analytical ultracentrifugation for analysis of doxorubicin loaded liposomes. International Journal of Pharmaceutics, 2017, 523 (1), pp.320-326. ⟨10.1016/j.ijpharm.2017.03.046⟩. ⟨cea-02202414⟩
98 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More