ss-CD-Functionalized Microdevice for Rapid Capture and Release of Bacteria

Abstract : Most procedures for detecting pathogens in liquid media require an initial concentration step. In this regard, carbohydrates have proven to be attractive affinity ligands for the solid-phase capture of bacteria that use lectins for adhesion to host cell membranes. However, the use of cyclodextrin-immobilized substrates to selectively trap bacteria has not been explored before. Here, using, quartz-crystal microbalance with dissipation monitoring experiments, we demonstrate that functionalization of surfaces by ss-cydodextrin (ss-CD) can not only allow for rapid and efficient capture of bacterial cells in liquid but also their facile elution with an aqueous solution of a selectively methylated ss-CD derivative as a competitive molecule. This capture/ elution strategy, which is based on host guest interactions between membrane components of the bacterial cell and the. CD cavities, is performed in physiological conditions and can be integrated in a microchip. Indeed, proof-of-concept studies showed the potential of ss-CD-modified micropillar-integrated microfluidic devices for concentration of bacteria. The results obtained with Escherichia coli suggest that this approach could be broadly applicable among Gram-negative bacteria, which share common cell membrane structures.
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https://hal-cea.archives-ouvertes.fr/cea-02202409
Contributor : Marianne Leriche <>
Submitted on : Wednesday, July 31, 2019 - 4:33:29 PM
Last modification on : Friday, August 2, 2019 - 2:59:35 AM

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Alexandra Perez-Anes, Anna Szarpak-Jankowska, Dorothee Jary, Rachel Auzely-Velty. ss-CD-Functionalized Microdevice for Rapid Capture and Release of Bacteria. ACS Applied Materials & Interfaces, Washington, D.C. : American Chemical Society, 2017, 9 (16), pp.13928-13938. ⟨10.1021/acsami.7b02194⟩. ⟨cea-02202409⟩

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