Skip to Main content Skip to Navigation
Journal articles

Curvature-dependent constraints drive remodeling of epithelia

Abstract : Epithelial tissues function as barriers that separate the organism from the environment. They usually have highly curved shapes, such as tubules or cysts. However, the processes by which the geometry of the environment and the cell's mechanical properties set the epithelium shape are not yet known. In this study, we encapsulated two epithelial cell lines, MDCK and J3B1A, into hollow alginate tubes and grew them under cylindrical confinement forming a complete monolayer. MDCK monolayers detached from the alginate shell at a constant rate, whereas J3B1A monolayers detached at a low rate unless the tube radius was reduced. We showed that this detachment is driven by contractile stresses in the epithelium and can be enhanced by local curvature. This allows us to conclude that J3B1A cells exhibit smaller contractility than MDCK cells. Monolayers inside curved tubes detach at a higher rate on the outside of a curve, confirming that detachment is driven by contraction.
Document type :
Journal articles
Complete list of metadata
Contributor : Marianne Leriche Connect in order to contact the contributor
Submitted on : Wednesday, July 17, 2019 - 12:22:04 PM
Last modification on : Friday, September 30, 2022 - 11:28:09 AM

Links full text




Florian A. Maechler, Cedric Allier, Aurelien Roux, Caterina Tomba. Curvature-dependent constraints drive remodeling of epithelia. Journal of Cell Science, Company of Biologists, 2019, 132 (4), ⟨10.1242/jcs.222372⟩. ⟨cea-02186468⟩



Record views