Large field-of-view phase and fluorescence mesoscope with microscopic resolution

Isaure de Kernier; Anais Ali-Cherif; Nelly Rongeat; Olivier Cioni; Sophie Morales; Julien Savatier; Serge Monneret; Pierre Blandin

Large field-of-view phase and fluorescence mesoscope with microscopic resolution

Isaure de Kernier ^{1, 2} Anais Ali-Cherif ³ Nelly Rongeat ³ Olivier Cioni ² Sophie Morales ² Julien Savatier ¹ Serge Monneret ¹ Pierre Blandin ²

1 FRESNEL - Institut FRESNEL

2 CEA-LETI - Laboratoire d'Electronique et des Technologies de l'Information

3 HORIBA ABX SAS - HORIBA Medical

Abstract : Phase and fluorescence are complementary contrasts that are commonly used in biology. However, the coupling of these two modalities is traditionally limited to high magnification and complex imaging systems. For statistical studies of biological populations, a large field-of-view is required. We describe a 30 mm(2) field-of-view dual-modality mesoscope with a 4-mu m resolution. The potential of the system to address biological questions is illustrated on white blood cell numeration in whole blood and multiwavelength imaging of the human osteosarcoma (U2-OS) cells. (C) The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License.

Keywords : fluorescence phase in-line holography microscopy

Document type :

Journal articles

Domain :

Engineering Sciences [physics]

Complete list of metadatas

https://hal-cea.archives-ouvertes.fr/cea-02186442
Contributor : Marianne Leriche <>
Submitted on : Wednesday, July 17, 2019 - 12:21:15 PM
Last modification on : Wednesday, July 31, 2019 - 1:24:36 AM

Large field-of-view phase and fluorescence mesoscope with microscopic resolution

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Large field-of-view phase and fluorescence mesoscope with microscopic resolution

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