Large field-of-view phase and fluorescence mesoscope with microscopic resolution

Isaure de Kernier (1, 2) , Anais Ali-Cherif (3) , Nelly Rongeat (3) , Olivier Cioni (2) , Sophie Morales (2) , Julien Savatier (1) , Serge Monneret (1) , Pierre Blandin (2)

1 FRESNEL - Institut FRESNEL
2 CEA-LETI - Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information
3 HORIBA ABX SAS - HORIBA Medical

Phase and fluorescence are complementary contrasts that are commonly used in biology. However, the coupling of these two modalities is traditionally limited to high magnification and complex imaging systems. For statistical studies of biological populations, a large field-of-view is required. We describe a 30 mm(2) field-of-view dual-modality mesoscope with a 4-mu m resolution. The potential of the system to address biological questions is illustrated on white blood cell numeration in whole blood and multiwavelength imaging of the human osteosarcoma (U2-OS) cells.

Keywords: fluorescence; phase; in-line holography; microscopy; in-line holography Paper 180667R

Domains: Engineering Sciences [physics]

Isaure de Kernier, Anais Ali-Cherif, Nelly Rongeat, Olivier Cioni, Sophie Morales, et al.. Large field-of-view phase and fluorescence mesoscope with microscopic resolution. Journal of Biomedical Optics, 2019, 24 (3), pp.036501. ⟨10.1117/1.JBO.24.3.036501⟩. ⟨cea-02186442⟩