Large field-of-view phase and fluorescence mesoscope with microscopic resolution

Abstract : Phase and fluorescence are complementary contrasts that are commonly used in biology. However, the coupling of these two modalities is traditionally limited to high magnification and complex imaging systems. For statistical studies of biological populations, a large field-of-view is required. We describe a 30 mm(2) field-of-view dual-modality mesoscope with a 4-mu m resolution. The potential of the system to address biological questions is illustrated on white blood cell numeration in whole blood and multiwavelength imaging of the human osteosarcoma (U2-OS) cells.
Document type :
Journal articles
Complete list of metadatas

Cited literature [48 references]  Display  Hide  Download

https://hal-cea.archives-ouvertes.fr/cea-02186442
Contributor : Marianne Leriche <>
Submitted on : Sunday, November 24, 2019 - 7:00:55 PM
Last modification on : Tuesday, November 26, 2019 - 2:06:11 PM

File

036501_1-2.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Isaure de Kernier, Anais Ali-Cherif, Nelly Rongeat, Olivier Cioni, Sophie Morales, et al.. Large field-of-view phase and fluorescence mesoscope with microscopic resolution. Journal of Biomedical Optics, Society of Photo-optical Instrumentation Engineers, 2019, 24 (3), pp.036501. ⟨10.1117/1.JBO.24.3.036501⟩. ⟨cea-02186442⟩

Share

Metrics

Record views

81

Files downloads

42