Evidence for a “metabolically inactive” inorganic phosphate pool in adenosine triphosphate synthase reaction using localized 31P saturation transfer magnetic resonance spectroscopy in the rat brain at 11.7 T

Brief Communication

Brice Tiret (1, 2) , Emmanuel Brouillet (1, 2) , Julien Valette (1, 2)

1 LMN - Laboratoire des Maladies Neurodégénératives - UMR 9199
2 Molecular Imaging Research Center, Fontenay aux Roses

With the increased spectral resolution made possible at high fields, a second, smaller inorganic phosphate resonance can be resolved on $^{31}$P magnetic resonance spectra in the rat brain. Saturation transfer was used to estimate de $novo$ adenosine triphosphate synthesis reaction rate. While the main inorganic phosphate pool is used by adenosine triphos-phate synthase, the second pool is inactive for this reaction. Accounting for this new pool may not only help us understand $^{31}$P magnetic resonance spectroscopy metabolic profiles better but also better quantify adenosine triphos-phate synthesis.

Keywords: Mathematical modelling; energy metabolism; adenosine triphosphate; pH; magnetic resonance spectroscopy

Domains: Life Sciences [q-bio]; Neurons and Cognition [q-bio.NC]

Brice Tiret, Emmanuel Brouillet, Julien Valette. Evidence for a “metabolically inactive” inorganic phosphate pool in adenosine triphosphate synthase reaction using localized 31P saturation transfer magnetic resonance spectroscopy in the rat brain at 11.7 T. Journal of Cerebral Blood Flow and Metabolism, 2016, 36 (9), pp.1513-1518. ⟨10.1177/0271678X16657095⟩. ⟨cea-02155315⟩