Evaluation of a new method for the collection and measurement of 8-isoprostane in exhaled breath for future application in nanoparticle exposure biomonitoring

Caroline Marie-Desvergne (1) , Muriel Dubosson (1) , Véronique Chamel Mossuz (1)

1 NPS - NanoSafety Platform

Background. In the field of nanoparticle exposure biomonitoring, oxidative stress biomarkers measured in exhaled breath condensate appear promising to detect early respiratory effects in workers handling nanomaterials. However, condensation is known for its poor efficiency in collecting non-volatiles in exhaled breath, leading to the low sensitivity of such measurements. Moreover, to be easily used in field studies on large groups of workers, the collection device must be disposable and convenient. Objectives. In this study, we have tested a totally disposable commercial device that allows for the easy dry collection of exhaled air after filtration on a patented filter. The suitability and efficiency of the SensAbues (SB) device for collecting 8-isoprostane were evaluated and compared to the RTube (RT). Methods. Seven healthy volunteers performed two 15 min collections of exhaled breath, one with the SB and one with the RT. Blank devices were used to determine the background levels induced by each device. 8-isoprostane was measured in all samples using an EIA technique. Results. The levels of 8-isoprostane in the exhaled breath of volunteers after collection with the SB were significantly higher than those after collection with the RT. Moreover, the levels obtained in volunteers with the SB were significantly higher than background levels obtained in blank devices, which was not the case for the RT. Conclusions. This is the first study to report the ability of the SB device to collect and measure 8-isoprostane in exhaled breath. The proposed method offers better sensitivity than a classical collection with the RT device and should be further explored before future application in biomonitoring studies. List of abbreviations EBC exhaled breath condensate EIA(s) enzyme immunoassay(s) NP(s) nanoparticle(s) RT RTube SB SensAbues

Mots clés: 8-isoprostane; RTube; exhaled breath condensate; SensAbues

Domaines: Sciences du Vivant [q-bio]

Caroline Marie-Desvergne, Muriel Dubosson, Véronique Chamel Mossuz. Evaluation of a new method for the collection and measurement of 8-isoprostane in exhaled breath for future application in nanoparticle exposure biomonitoring. Journal of Breath Research, 2018, 12 (3), pp.031001. ⟨10.1088/1752-7163/aabdf2⟩. ⟨cea-02150765⟩