Skip to Main content Skip to Navigation
Journal articles

The food additive E171 and titanium dioxide nanoparticles indirectly alter the homeostasis of the human intestinal epithelial cells in vitro

Marie Dorier 1 David Béal 1 Céline Tisseyre 1 Caroline Marie-Desvergne 2 Muriel Dubosson 2 Frédérick Barreau 3 Eric Houdeau 4 Nathalie Herlin-Boime 5 Thierry Rabilloud 6 Marie Carrière 1, * 
* Corresponding author
1 CIBEST - Chimie Interface Biologie pour l’Environnement, la Santé et la Toxicologie
SYMMES - SYstèmes Moléculaires et nanoMatériaux pour l’Energie et la Santé : DRF/IRIG/SYMMES
5 LEDNA - Laboratoire Edifices Nanométriques
NIMBE UMR 3685 - Nanosciences et Innovation pour les Matériaux, la Biomédecine et l'Energie (ex SIS2M)
6 ProMD - Protéomique, Métaux et Différenciation
LCBM - UMR 5249 - Laboratoire de Chimie et Biologie des Métaux
Abstract : Environmental contamination with TiO2 and the use of TiO2 as a food additive (E171) or in cosmetics resultin human exposure to TiO2 via inhalation, ingestion, and through skin contact. When inhaled, most TiO2particles are cleared via the mucociliary escalator and are then swallowed. Together with the ingestion ofE171, this process results in a significant exposure of the human gastro-intestinal tract to TiO2. One of thefunctions of the intestine is to protect the body from external aggression, via the so-called intestinal barrierfunction. The aim of this study was to determine whether, and through which mechanisms, TiO2 affectsthis function. Caco-2 and HT29-MTX cells were co-cultured to reconstitute an in vitro mucus-secreting intestinal epithelium. This epithelium was exposed to TiO2-NPs, either pure anatase or mixed anatase/rutile, or to E171. Two exposure scenarii were used: acute exposure for 6 h or 48 h after cell differentiation (21 days post-seeding), or repeated exposure during the course of cell differentiation, i.e., twice a week for 21 days post-seeding. Epithelial cells repeatedly exposed to TiO2 developed an inflammatory profile, together with increased mucus secretion. Epithelial integrity was unaltered, but the content of ATP-binding cassette (ABC) family xenobiotic efflux pumps was modified. Taken together, these data show that TiO2 moderatelybut significantly dysregulates several features that contribute to the protective function of the intestine.
Complete list of metadata

Cited literature [66 references]  Display  Hide  Download
Contributor : Serge Palacin Connect in order to contact the contributor
Submitted on : Monday, May 6, 2019 - 9:24:33 AM
Last modification on : Monday, July 4, 2022 - 8:53:14 AM
Long-term archiving on: : Wednesday, October 2, 2019 - 12:04:28 AM


Publisher files allowed on an open archive


Distributed under a Creative Commons Attribution - NonCommercial 4.0 International License



Marie Dorier, David Béal, Céline Tisseyre, Caroline Marie-Desvergne, Muriel Dubosson, et al.. The food additive E171 and titanium dioxide nanoparticles indirectly alter the homeostasis of the human intestinal epithelial cells in vitro. Environmental science‎.Nano, Royal Society of Chemistry, 2019, 6 (5), pp.1549-1561. ⟨10.1039/c8en01188e⟩. ⟨cea-02101713⟩



Record views


Files downloads