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, Study 2 combines an multimodal imaging approach using [ 18 F]DPA-714 PET and T2w and diffusion weighted MRI at week 5, 7 and 9 p.i. In both studies mice were euthanized (?) after the last imaging examination at week 9, Supplementary Figure S1: Study design for the PET and MRI acquisitions. Study 1 comprises a longitudinal PET study using

. Additionally, Among these animals, n=5 animals also underwent [ 18 F]FET PET 24 hours prior to [ 18 F]DPA-714 PET acquisitions at 7 weeks and n=2 mice at 9 weeks p.i., respectively. Supplementary Figure S2: Ipsi-to-contralateral or ipsilateral-to-corpus callosum SUV ratios at different imaging points (A) (from 1 to 9 weeks post implantation, A significant increase of ipsi-to, vol.7

, 01) p.i., respectively, indicating a significant increase of tracer uptake in the ipsilateral tumor bearing hemisphere. Ipsilateral-to-corpus-callosum ratios do not increase significantly over time, but demonstrate decreased values compared to ipsi-to-contralateral ratios. A significant decreased ratio is calculated at 9 weeks p.i., indicating increased tracer uptake from

, Volumetric analysis of PET tracer uptake over time (B). Illustration of tracer volumes fused on a T2w MRI template (upper part)

*. ,

, or mouse TSPO (mTSPO) in coronal slice of a mouse brain at 9 weeks post glioma cell injection. A Merged overview scan and B higher magnification images of hTSPO (green) and CD11b (red) signal in different tumor-related zones as the tumor core (tc), the migratory zone at the corpus callosum (cc) and an infiltrative zone (iz) at the tumor border. C Merged overview scan and D higher magnification images of mTSPO (green) and CD11b (red) signal in similar zone as described above, Supplementary Figure S3: Immunohistochemical staining of CD11b and human (hTSPO)

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