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Journal articles
Structural adaptations of photosynthetic complex I enable ferredoxin-dependent electron transfer
Jan M. Schuller
1
James A. Birrell
2
Hideaki Tanaka
3
Tsuyoshi Konuma
4
Hannes Wulfhorst
5
Nicholas Cox
2
Sandra K. Schuller
6
Jacqueline Thiemann
5
Wolfgang Lubitz
2
Pierre Sétif
7
Takahisa Ikegami
8
Benjamin D. Engel
1
Genji Kurisu
3
Marc M. Nowaczyk
Hannes Wulfhorst 5
Author
Nicholas Cox 2
Author
Sandra K. Schuller 6
Author
Jacqueline Thiemann 5
Author
Genji Kurisu 3
Author
Marc M. Nowaczyk
Author
1
MPIB - Max Planck Institute of Biochemistry (Am Klopferspitz 18, 82152 Martinsried, Germany - Germany)
- Max-Planck-Gesellschaft (Jägerstrasse, 10-11, D-10117 Berlin - Germany)
2
Max Planck Institute for Chemical Energy Conversion (Stiftstr. 34 - 36, 45470 Mülheim an der Ruhr - Germany)
- Max-Planck-Gesellschaft (Jägerstrasse, 10-11, D-10117 Berlin - Germany)
3
Institute for Protein Research [Osaka] (3-2 Yamadaoka, Suita-shi, Osaka 565-0871 - Japan)
- Osaka University [Osaka] (3-3-138 Sugimoto Sumiyoshi-ku, Osaka 558-8585 - Japan)
4
YCUSM - Yokohama City University School of Medecine (Fukuura Kanazawa-ku - Japan)
5
RUB - Ruhr University Bochum (Universitätsstraße 150, 44801 Bochum - Germany)
6
LMU - Ludwig-Maximilians-Universität München (Geschwister-Scholl-Platz 1, 80539 München - Germany)
7
I2BC - Institut de Biologie Intégrative de la Cellule (Bâtiment 21, 1 avenue de la Terrasse, 91198 Gif/Yvette cedex - France)
- CEA - Commissariat à l'énergie atomique et aux énergies alternatives : DRF/I2BC (Centre de Saclay Centre de Grenoble Centre de Cadarache etc - France)
- Université Paris-Saclay (Espace Technologique, Bat. Discovery - RD 128 - 2e ét., 91190 Saint-Aubin - France)
- CNRS - Centre National de la Recherche Scientifique : UMR 9198 (France)
8
YCU - Yokohama City University (22-2 Seto, Kanazawa Ward, Yokohama, Kanagawa 236-0027 - Japan)
Abstract : Photosynthetic complex I enables cyclic electron flow around photosystem I, a regulatory mechanism for photosynthetic energy conversion. We report a 3.3-angstrom-resolution cryo–electron microscopy structure of photosynthetic complex I from the cyanobacterium Thermosynechococcus elongatus. The model reveals structural adaptations that facilitate binding and electron transfer from the photosynthetic electron carrier ferredoxin. By mimicking cyclic electron flow with isolated components in vitro, we demonstrate that ferredoxin directly mediates electron transfer between photosystem I and complex I, instead of using intermediates such as NADPH (the reduced form of nicotinamide adenine dinucleotide phosphate). A large rate constant for association of ferredoxin to complex I indicates efficient recognition, with the protein subunit NdhS being the key component in this process.
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https://hal-cea.archives-ouvertes.fr/cea-02065421
Contributor : Bruno Savelli Connect in order to contact the contributor
Submitted on : Tuesday, March 12, 2019 - 4:31:40 PM
Last modification on : Wednesday, August 25, 2021 - 4:38:02 PM
Contributor : Bruno Savelli Connect in order to contact the contributor
Submitted on : Tuesday, March 12, 2019 - 4:31:40 PM
Last modification on : Wednesday, August 25, 2021 - 4:38:02 PM
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- HAL Id : cea-02065421, version 1
- DOI : 10.1126/science.aau3613
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CEA | CNRS | CEA-UPSAY | I2BC | CEA-DRF | JOLIOT | GS-ENGINEERING | GS-BIOSPHERA | INSTITUT-SCIENCES-LUMIERE | GS-HEALTH-DRUG-SCIENCES
Citation
Jan M. Schuller, James A. Birrell, Hideaki Tanaka, Tsuyoshi Konuma, Hannes Wulfhorst, et al.. Structural adaptations of photosynthetic complex I enable ferredoxin-dependent electron transfer. Science, American Association for the Advancement of Science, 2019, 363 (6424), pp.257-260. ⟨10.1126/science.aau3613⟩. ⟨cea-02065421⟩
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