Current Developments in $\mu$MAS NMR Analysis for Metabolomics

Covadonga Lucas-Torres 1 Alan Wong 1, *
* Corresponding author
1 LSDRM - Laboratoire Structure et Dynamique par Résonance Magnétique (LCF)
NIMBE UMR 3685 - Nanosciences et Innovation pour les Matériaux, la Biomédecine et l'Energie (ex SIS2M)
Abstract : Analysis of microscopic specimens has emerged as a useful analytical application in metabolomics because of its capacity for characterizing a highly homogenous sample with a specific interest. The undeviating analysis helps to unfold the hidden activities in a bulk specimen and contributes to the understanding of the fundamental metabolisms in life. In NMR spectroscopy, micro($\mu$)-probe technology is well-established and-adopted to the microscopic level of biofluids. However, this is quite the contrary with specimens such as tissue, cell and organism. This is due to the substantial difficulty of developing a sufficient $\mu$-size magic-angle spinning (MAS) probe for sub-milligram specimens with the capability of high-quality data acquisition. It was not until 2012; a $\mu$MAS probe had emerged and shown promises to $\mu$g analysis; since, a continuous advancement has been made striving for the possibility of $\mu$MAS to be an effective NMR spectroscopic analysis. Herein, the mini-review highlights the progress of $\mu$MAS development-from an impossible scenario to an attainable solution-and describes a few demonstrative metabolic profiling studies. The review will also discuss the current challenges in $\mu$MAS NMR analysis and its potential to metabolomics.
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Covadonga Lucas-Torres, Alan Wong. Current Developments in $\mu$MAS NMR Analysis for Metabolomics. Metabolites, MDPI, 2019, 9 (2), pp.29. ⟨https://doi.org/10.3390/metabo9020029⟩. ⟨10.3390/metabo9020029⟩. ⟨cea-02017088⟩

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