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Simultaneous metabolic mapping of different anatomies by $^1$H HR-MAS chemical shift imaging

Alan Wong 1 Covadonga Lucas-Torres 1 
1 LSDRM - Laboratoire Structure et Dynamique par Résonance Magnétique (LCF)
NIMBE UMR 3685 - Nanosciences et Innovation pour les Matériaux, la Biomédecine et l'Energie (ex SIS2M)
Abstract : Localized information on a specimen is considered indispensable for deciphering biological activity. Magnetic resonance spectroscopy is a notable method because of its versatility; however, one limitation is the spectral quality on a static sample. This study explores an amalgamated method with two magnetic resonance experiments: high-resolution magic-angle spinning (HR-MAS) for high-quality spectral acquisition from a spinning sample and chemical shift imaging (CSI) for spatial localization. The advantage of HR-MAS CSI is its amenity for simultaneously profiling the metabolome—with good spectral data—at different spatial regions in a single experiment. Herein, $^1$H HR-MAS CSI (including a T$_2$-contrast CSI) was described and performed on various food tissues and an intact organism. Different data analyses such as multivariate and quantification were explored to identify the metabolic variants in different anatomical regions and in one case, to assist in a spatial allocation. The limitation and drawback of the experiment are also discussed.
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Submitted on : Tuesday, February 5, 2019 - 7:26:53 AM
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Alan Wong, Covadonga Lucas-Torres. Simultaneous metabolic mapping of different anatomies by $^1$H HR-MAS chemical shift imaging. Analytical and Bioanalytical Chemistry, 2019, 411, pp.1591-1599. ⟨10.1007/s00216-019-01603-w⟩. ⟨cea-02006983⟩



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