Proteogenomic insights into uranium tolerance of a Chernobyl's $Microbacterium$ bacterial isolate

Nicolas Gallois; Béatrice Alpha-Bazin; Philippe Ortet; Mohamed Barakat; Laurie Piette; Justine Long; Catherine Berthomieu; Jean Armengaud; Virginie Chapon

doi:10.1016/j.jprot.2017.11.021

Proteogenomic insights into uranium tolerance of a Chernobyl's $Microbacterium$ bacterial isolate

Nicolas Gallois ^{1, 2} Béatrice Alpha-Bazin ³ Philippe Ortet ^{1, 4} Mohamed Barakat ^{1, 4} Laurie Piette ^{1, 2} Justine Long ^{1, 4} Catherine Berthomieu ^{1, 2} Jean Armengaud ^{3, *} Virginie Chapon ^{1, 2}

* Corresponding author

1 BIAM - Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB)

2 IPM - Interactions Protéine Métal

BIAM - Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) : DRF/BIAM

3 LI2D - Laboratoire Innovations technologiques pour la Détection et le Diagnostic

SPI - Service de Pharmacologie et Immunoanalyse : DRF/JOLIOT

4 LEMIRE - Laboratoire d'Ecologie Microbienne de la Rhizosphère et d'Environnements Extrêmes

BIAM - Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) : DRF/BIAM

Abstract : $Microbacterium$ $oleivorans$ A9 is a uranium-tolerant actinobacteria isolated from the trench T22 located near the Chernobyl nuclear power plant. This site is contaminated with different radionuclides including uranium. To observe the molecular changes at the proteome level occurring in this strain upon uranyl exposure and understand molecular mechanisms explaining its uranium tolerance, we established its draft genome and used this raw information to perform an in-depth proteogenomics study. High-throughput proteomics were performed on cells exposed or not to 10$\mu$M uranyl nitrate sampled at three previously identified phases of uranyl tolerance. We experimentally detected and annotated 1532 proteins and highlighted a total of 591 proteins for which abundances were significantly differing between conditions. Notably, proteins involved in phosphate and iron metabolisms show high dynamics. A large ratio of proteins more abundant upon uranyl stress, are distant from functionally-annotated known proteins, highlighting the lack of fundamental knowledge regarding numerous key molecular players from soil bacteria.

Keywords : Toxicoproteomics Microbacterium Radionuclides Toxicity Proteogenomics Bioinformatics

Document type :

Journal articles

Domain :

Life Sciences [q-bio]

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https://hal-cea.archives-ouvertes.fr/cea-01944196
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Submitted on : Wednesday, December 5, 2018 - 11:47:43 AM
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