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Journal Articles EMBO Journal Year : 2001

Characterisation of homologous recombination induced by replication inhibition in mammalian cells

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Abstract

To analyze relationships between replication and homologous recombination in mammalian cells, we used replication inhibitors to treat mouse and hamster cell lines containing tandem repeat recombination substrates. In the ®rst step, few double-strand breaks (DSBs) are produced, recombination is slightly increased, but cell lines defective in non-homologous end-joining (NHEJ) affected in ku86 (xrs6) or xrcc4 (XR-1) genes show enhanced sensitivity to replication inhibitors. In the second step, replication inhibition leads to coordinated kinetics of DSB accumulation, Rad51 foci formation and RAD51-dependent gene conversion stimulation. In xrs6 as well as XR-1 cell lines, Rad51 foci accumulate more rapidly compared with their respective controls. We propose that replication inhibition produces DSBs, which are ®rst processed by the NHEJ; then, following DSB accumulation, RAD51 recombination can act.
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cea-01938127 , version 1 (28-11-2018)

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Yannick Saintigny, Fabien Delacote, Guillaume Vares, Fabrice Petitot, Sarah Lambert, et al.. Characterisation of homologous recombination induced by replication inhibition in mammalian cells. EMBO Journal, 2001, 20 (14), pp.3861-3870. ⟨10.1093/emboj/20.14.3861⟩. ⟨cea-01938127⟩
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