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Single bacteria identification by Raman spectroscopy

Abstract : We report on rapid identification of single bacteria using a low-cost, compact, Raman spectroscope. We demonstrate that a 60-s procedure is sufficient to acquire a comprehensive Raman spectrum in the range of 600 to 3300 cm−1. This time includes localization of small bacteria aggregates, alignment on a single individual, and spontaneous Raman scattering signal collection. Fast localization of small bacteria aggregates, typically composed of less than a dozen individuals, is achieved by lensfree imaging over a large field of view of 24 mm2. The lensfree image also allows precise alignment of a single bacteria with the probing beam without the need for a standard microscope. Raman scattered light from a 34-mW continuous laser at 532 nm was fed to a customized spectrometer (prototype Tornado Spectral Systems). Owing to the high light throughput of this spectrometer, integration times as low as 10 s were found acceptable. We have recorded a total of 1200 spectra over seven bacterial species. Using this database and an optimized preprocessing, classification rates of ∼90% were obtained. The speed and sensitivity of our Raman spectrometer pave the way for high-throughput and nondestructive real-time bacteria identification assays. This compact and low-cost technology can benefit biomedical, clinical diagnostic, and environmental applications.
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https://hal-cea.archives-ouvertes.fr/cea-01895995
Contributor : Marie-France Robbe <>
Submitted on : Monday, October 15, 2018 - 4:40:07 PM
Last modification on : Thursday, June 11, 2020 - 5:04:07 PM

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Samy Andrea Strola, Jean-Charles Baritaux, Emmanuelle Schultz, Anne-Catherine Simon, Cédric Allier, et al.. Single bacteria identification by Raman spectroscopy. Journal of Biomedical Optics, Society of Photo-optical Instrumentation Engineers, 2014, 19 (11), pp.111610. ⟨10.1117/1.JBO.19.11.111610⟩. ⟨cea-01895995⟩

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