Epigenome-wide DNA methylation profiling in Progressive Supranuclear Palsy reveals major changes at DLX1

Axel Weber (1) , Sigrid C. Schwarz (2, 3) , Jorg Tost (4, 5) , Dietrich Trümbach (6, 7) , Pia Winter (1) , Florence Busato (5, 4) , Pawel Tacik (8) , Anita Windhorst (1) , Maud Fagny (4, 5) , Thomas Arzberger (3) , Catriona Mclean (9) , John van Swieten (10) , Johannes Schwarz (2) , Daniela Vogt Weisenhorn (3, 7) , Wolfgang Wurst (3, 7) , Till Adhikary (11) , Dennis Dickson (8) , Günter Höglinger (2, 3) , Ulrich Müller (1)

1 JLU - Justus-Liebig-Universität Gießen = Justus Liebig University
2 TUM - Technische Universität Munchen - Technical University Munich - Université Technique de Munich
3 DZNE - University of Munich
4 CNG - Centre National de Génotypage
5 JACOB - Institut de Biologie François JACOB
6 Biochemistry
7 Institute of Developmental Genetics, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt, Lehrstuhl für Entwicklungsgenetik,
8 Mayo Clinic [Jacksonville]
9 State Neuropathology Service, Department of Pathology
10 Erasmus MC - Erasmus University Medical Center [Rotterdam]
11 Philipps Universität Marburg = Philipps University of Marburg

Genetic, epigenetic, and environmental factors contribute to the multifactorial disorder progressive supranuclear palsy (PSP). Here, we study epigenetic changes by genome-wide analysis of DNA from postmortem tissue of forebrains of patients and controls and detect significant (P < 0.05) methylation differences at 717 CpG sites in PSP vs. controls. Four-hundred fifty-one of these sites are associated with protein-coding genes. While differential methylation only affects a few sites in most genes, DLX1 is hypermethylated at multiple sites. Expression of an antisense transcript of DLX1, DLX1AS, is reduced in PSP brains. The amount of DLX1 protein is increased in gray matter of PSP forebrains. Pathway analysis suggests that DLX1 influences MAPT-encoded Tau protein. In a cell system, overexpression of DLX1 results in downregulation of MAPT while overexpression of DLX1AS causes upregulation of MAPT. Our observations suggest that altered DLX1 methylation and expression contribute to pathogenesis of PSP by influencing MAPT

Domaines: Sciences du Vivant [q-bio]

Axel Weber, Sigrid C. Schwarz, Jorg Tost, Dietrich Trümbach, Pia Winter, et al.. Epigenome-wide DNA methylation profiling in Progressive Supranuclear Palsy reveals major changes at DLX1. Nature Communications, 2018, 9, pp.2929. ⟨10.1038/s41467-018-05325-y⟩. ⟨cea-01877684⟩