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Journal articles
A mechanism for CO regulation of ion channels
Sofia M. Kapetanaki
1
Mark J. Burton
2
Jaswir Basran
2
Chiasa Uragami
3
Peter C. E. Moody
2
John S. Mitcheson
2
Ralf Schmid
2
Noel W. Davies
2
Pierre Dorlet
3
Marten H. Vos
4
Nina M. Storey
2
Emma Raven
1
Pierre Dorlet 3
Author IdHAL : pierre-dorlet ORCID : https://orcid.org/0000-0001-7394-3374
Marten H. Vos 4
Author IdHAL : marten-h-vos ORCID : https://orcid.org/0000-0003-0493-4831
Nina M. Storey 2
Author
Emma Raven 1
Author
1
Department of Chemistry [Leicester] (University of Leicester,
Leicester, LE1 7RH - United Kingdom)
- University of Leicester (University Rd, Leicester LE1 7RH - United Kingdom)
2
Department of Molecular and Cell Biology [Leicester] (University of Leicester - United Kingdom)
- University of Leicester (University Rd, Leicester LE1 7RH - United Kingdom)
3
I2BC - Institut de Biologie Intégrative de la Cellule (Bâtiment 21, 1 avenue de la Terrasse, 91198 Gif/Yvette cedex - France)
- CEA - Commissariat à l'énergie atomique et aux énergies alternatives : DRF/I2BC (Centre de Saclay Centre de Grenoble Centre de Cadarache etc - France)
- Université Paris-Saclay (Espace Technologique, Bat. Discovery - RD 128 - 2e ét., 91190 Saint-Aubin - France)
- CNRS - Centre National de la Recherche Scientifique : UMR 9198 (France)
4
LOB - Laboratoire d'Optique et Biosciences (Ecole Polytechnique
91128 PALAISEAU Cedex - France)
- X - École polytechnique (École polytechnique, 91128 Palaiseau Cedex - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1182 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMR7645 (France)
Abstract : Despite being highly toxic, carbon monoxide (CO) is also an essential intracellular signalling molecule. The mechanisms of CO-dependent cell signalling are poorly defined, but are likely to involve interactions with heme proteins. One such role for CO is in ion channel regulation. Here, we examine the interaction of CO with K$_{ATP}$ channels. We find that CO activates K$_{ATP}$ channels and that heme binding to a CXXHX$_{16}$H motif on the SUR2A receptor is required for the CO-dependent increase in channel activity. Spectroscopic and kinetic data were used to quantify the interaction of CO with the ferrous heme-SUR2A complex. The results are significant because they directly connect CO-dependent regulation to a heme-binding event on the channel. We use this information to present molecular-level insight into the dynamic processes that control the interactions of CO with a heme-regulated channel protein, and we present a structural framework for understanding the complex interplay between heme and CO in ion channel regulation.
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Journal articles
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https://hal-cea.archives-ouvertes.fr/cea-01876610
Contributor : Bruno Savelli <>
Submitted on : Tuesday, September 18, 2018 - 3:41:31 PM
Last modification on : Thursday, July 8, 2021 - 3:51:12 AM
Contributor : Bruno Savelli <>
Submitted on : Tuesday, September 18, 2018 - 3:41:31 PM
Last modification on : Thursday, July 8, 2021 - 3:51:12 AM
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- HAL Id : cea-01876610, version 1
Collections
CEA | CNRS | X-DEP-BIO | X-LOB | CEA-UPSAY | X | CEA-DRF | I2BC | JOLIOT | GS-ENGINEERING | GS-BIOSPHERA | INSTITUT-SCIENCES-LUMIERE | GS-HEALTH-DRUG-SCIENCES
Citation
Sofia M. Kapetanaki, Mark J. Burton, Jaswir Basran, Chiasa Uragami, Peter C. E. Moody, et al.. A mechanism for CO regulation of ion channels. Nature Communications, Nature Publishing Group, 2018, 9, pp.907. ⟨cea-01876610⟩
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