Cell micro-irradiation with MeV protons counted by an ultra-thin diamond membrane

Abstract : We report the development of thin single crystal diamond membranes suitable for dose control in targeted cell irradiation experiments with a proton microbeam. A specific design was achieved to deliver single protons with a hit detection efficiency approaching 100%. The membranes have thicknesses between 1.8 and 3 pm and arc used as vacuum windows on the microbeam line. The impact of these transmission detectors on the microbeam spot size is estimated by Monte-Carlo simulations, indicating that a beam lateral resolution below 2 µm is achieved. This is confirmed by experiments showing the accumulation online of X-ray Repair Cross-Complementing protein 1 (XRCC1)-Green Fluorescent Protein (GFP) at DNA damaged sites in living cells.
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https://hal-cea.archives-ouvertes.fr/cea-01874782
Contributor : Marie-France Robbe <>
Submitted on : Friday, September 14, 2018 - 4:52:06 PM
Last modification on : Thursday, August 22, 2019 - 11:16:01 AM

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Philippe Barberet, Michal Pomorski, Giovanna Muggiolu, Eva Torfeh, Gérard Claverie, et al.. Cell micro-irradiation with MeV protons counted by an ultra-thin diamond membrane. Applied Physics Letters, American Institute of Physics, 2017, 111 (24), pp.243701. ⟨10.1063/1.5009713⟩. ⟨cea-01874782⟩

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