Cell micro-irradiation with MeV protons counted by an ultra-thin diamond membrane

Philippe Barberet 1 Michal Pomorski 2 Giovanna Muggiolu 1 Eva Torfeh 1 Gérard Claverie 1 Cédric Huss 1 Samuel Saada 2 Guillaume Devès 1 Marina Simon 1 Hervé Seznec 1
1 CENBG - Centre d'Etudes Nucléaires de Bordeaux Gradignan
2 LCD-LIST - Laboratoire Capteurs Diamant
DM2I - Département Métrologie Instrumentation & Information : DRT/LIST/DM2I
Abstract : We report the development of thin single crystal diamond membranes suitable for dose control in targeted cell irradiation experiments with a proton microbeam. A specific design was achieved to deliver single protons with a hit detection efficiency approaching 100%. The membranes have thicknesses between 1.8 and 3 pm and arc used as vacuum windows on the microbeam line. The impact of these transmission detectors on the microbeam spot size is estimated by Monte-Carlo simulations, indicating that a beam lateral resolution below 2 µm is achieved. This is confirmed by experiments showing the accumulation online of X-ray Repair Cross-Complementing protein 1 (XRCC1)-Green Fluorescent Protein (GFP) at DNA damaged sites in living cells.
Keywords : CHARGED-PARTICLE MICROBEAM SINGLE REPAIR RECRUITMENT
Document type :
Journal articles
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https://hal-cea.archives-ouvertes.fr/cea-01874782
Contributor : Marie-France Robbe <>
Submitted on : Friday, September 14, 2018 - 4:52:06 PM
Last modification on : Thursday, August 22, 2019 - 11:16:01 AM

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CEA | CENBG | TDS-MACS | CEA-UPSAY | DRT | IN2P3 | LIST | UNIV-PARIS-SACLAY | DM2I

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Philippe Barberet, Michal Pomorski, Giovanna Muggiolu, Eva Torfeh, Gérard Claverie, et al.. Cell micro-irradiation with MeV protons counted by an ultra-thin diamond membrane. Applied Physics Letters, American Institute of Physics, 2017, 111 (24), pp.243701. ⟨10.1063/1.5009713⟩. ⟨cea-01874782⟩

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