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Time-lapse contact microscopy of cell cultures based on non-coherent illumination

Abstract : Video microscopy offers outstanding capabilities to investigate the dynamics of biological and pathological mechanisms in optimal culture conditions. Contact imaging is one of the simplest imaging architectures to digitally record images of cells due to the absence of any objective between the sample and the image sensor. However, in the framework of in-line holography, other optical components, e.g., an optical filter or a pinhole, are placed underneath the light source in order to illuminate the cells with a coherent or quasi-coherent incident light. In this study, we demonstrate that contact imaging with an incident light of both limited temporal and spatial coherences can be achieved with sufficiently high quality for most applications in cell biology, including monitoring of cell sedimentation, rolling, adhesion, spreading, proliferation, motility, death and detachment. Patterns of cells were recorded at various distances between 0 and 1000 μm from the pixel array of the image sensors. Cells in suspension, just deposited or at mitosis focalise light into photonic nanojets which can be visualised by contact imaging. Light refraction by cells significantly varies during the adhesion process, the cell cycle and among the cell population in connection with every modification in the tridimensional morphology of a cell.
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Contributor : Marianne Leriche Connect in order to contact the contributor
Submitted on : Thursday, June 28, 2018 - 3:36:09 PM
Last modification on : Saturday, June 25, 2022 - 8:28:22 PM

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Marion Gabriel, Dorothée Balle, Stéphanie Bigault, Cyrille Pornin, Stéphane Gétin, et al.. Time-lapse contact microscopy of cell cultures based on non-coherent illumination. Scientific Reports, Nature Publishing Group, 2015, 5 (1), pp.14532. ⟨10.1038/srep14532⟩. ⟨cea-01825740⟩



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