The nano-bio interface mapped by oxidative footprinting of the adsorption sites of myoglobin - CEA - Commissariat à l’énergie atomique et aux énergies alternatives Accéder directement au contenu
Article Dans Une Revue Analytical and Bioanalytical Chemistry Année : 2014

The nano-bio interface mapped by oxidative footprinting of the adsorption sites of myoglobin

Résumé

Oxidative footprinting has been used to study the structure of macromolecular assemblies such as protein-protein and protein-ligand complexes. We propose a novel development of this technique to probe the protein corona that forms at the surface of nanoparticles in any biological medium. Indeed, very few techniques allow studying this interface at the molecular and residue level. Based on hydroxyl radical-mediated oxidation of proteins and analysis by nanoscale liquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS), two sites of adsorption of myoglobin on silica nanoparticles are identified. This method gives new insights in the understanding of protein adsorption on nanomaterials.
Fichier non déposé

Dates et versions

cea-01758749 , version 1 (04-04-2018)

Identifiants

Citer

Stephanie Devineau, Christelle Mathé, Véronique Legros, Florence Gonnet, Régis Daniel, et al.. The nano-bio interface mapped by oxidative footprinting of the adsorption sites of myoglobin. Analytical and Bioanalytical Chemistry, 2014, 406, pp.8037 - 8040. ⟨10.1007/s00216-014-8188-7⟩. ⟨cea-01758749⟩
100 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More