Altered enhancer transcription underlies Huntington’s disease striatal transcriptional signature - Archive ouverte HAL Access content directly
Journal Articles Scientific Reports Year : 2017

Altered enhancer transcription underlies Huntington’s disease striatal transcriptional signature

(1) , (1) , (2) , (2) , (3, 4) , (3, 4) , (2) , (2) , (2)
1
2
3
4

Abstract

Epigenetic and transcriptional alterations are both implicated in Huntington’s disease (HD), a progressive neurodegenerative disease resulting in degeneration of striatal neurons in the brain. However, how impaired epigenetic regulation leads to transcriptional dysregulation in HD is unclear. Here, we investigated enhancer RNAs (eRNAs), a class of long non-coding RNAs transcribed from active enhancers. We found that eRNAs are expressed from many enhancers of mouse striatum and showed that a subset of those eRNAs are deregulated in HD vs control mouse striatum. Enhancer regions producing eRNAs decreased in HD mouse striatum were associated with genes involved in striatal neuron identity. Consistently, they were enriched in striatal super-enhancers. Moreover, decreased eRNA expression in HD mouse striatum correlated with down-regulation of associated genes. Additionally, a significant number of RNA Polymerase II (RNAPII) binding sites were lost within enhancers associated with decreased eRNAs in HD vs control mouse striatum. Together, this indicates that loss of RNAPII at HD mouse enhancers contributes to reduced transcription of eRNAs, resulting in down-regulation of target genes. Thus, our data support the view that eRNA dysregulation in HD striatum is a key mechanism leading to altered transcription of striatal neuron identity genes, through reduced recruitment of RNAPII at super-enhancers
Fichier principal
Vignette du fichier
srep42875.pdf (1.23 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

cea-01751880 , version 1 (12-11-2021)

Licence

Attribution - CC BY 4.0

Identifiers

Cite

Stéphanie Le Gras, Céline Keime, Anne Anthony, Caroline Lotz, Lucie de Longprez, et al.. Altered enhancer transcription underlies Huntington’s disease striatal transcriptional signature. Scientific Reports, 2017, 7 (Article number: 42875), ⟨10.1038/srep42875⟩. ⟨cea-01751880⟩
63 View
30 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More