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Specific Recognition of G-Quadruplexes Over Duplex-DNA by a Macromolecular NIR Two-Photon Fluorescent Probe

Abstract : The implication of guanine-rich DNA sequences in biologically important roles such as telomerase dysfunction and the regulation of gene expression has prompted the search for structure-specific G-quadruplex agents for targeted diagnostic and therapeutic applications. Herein, we report on a near-infrared (NIR) two-photon poly(cationic) anthracene-based macromolecule able to selectively target G-quadruplexes (G4s) over genomic double-stranded DNA. In particular, the striking changes in its linear and third-order nonlinear optical properties, combined with the emergence of a strong induced electronic circular dichroism (ECD) signal upon binding to canonical and noncanonical DNA secondary structures allowed for a highly specific detection of several different G4s. Furthermore, through a detailed computational analysis we bring compelling evidence that our probe intercalation within G4s is a thermodynamically favored event, and we fully rationalize the spectroscopic evolution resulting from this complexation event by providing a reasonable explanation regarding the origin of the peculiar ECD effect that accompanies it.
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https://hal-cea.archives-ouvertes.fr/cea-01713521
Contributor : Caroline Lebe <>
Submitted on : Tuesday, February 20, 2018 - 4:27:34 PM
Last modification on : Monday, February 10, 2020 - 6:14:03 PM

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Marco Deiana, Bastien Mettra, Lara Martinez-Fernandez, Leszek M. Mazur, Krzysztof Pawlik, et al.. Specific Recognition of G-Quadruplexes Over Duplex-DNA by a Macromolecular NIR Two-Photon Fluorescent Probe. Journal of Physical Chemistry Letters, American Chemical Society, 2017, 8 (23), pp.5915 - 5920. ⟨10.1021/acs.jpclett.7b02547⟩. ⟨cea-01713521⟩

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